Moţa G, Mărgineanu M, Nicolae M, Bancu A, Moraru I
Department of Immunology, Victor Babes Institute, Bucharest, Romania.
Immunol Lett. 1988 May;18(1):67-72. doi: 10.1016/0165-2478(88)90072-7.
Antibodies of the IgG class, specifically interacted with H-2 antigens of murine leukemia EL4 cells, were used to bind the ricin toxin covalently linked to protein A of Staphylococcus aureus. The toxin thus complexed, introduced in the cytoplasm by endocytosis, was able to kill the leukemic cells inoculated in animals. The interaction of immunotoxin with the leukemic cells was performed in vitro using one, two or three treatments and the cytotoxic effect on the target cells was followed up in vivo. The time interval between immunotoxin treatments was indicated by the membrane turn-over study of EL4 cells coated with specific antibodies in their monomeric form, complexed by protein A or interacted with protein A--ricin toxin conjugate. A proportion of 99.8% cells killed was obtained after three treatments.
IgG类抗体与鼠白血病EL4细胞的H-2抗原特异性相互作用，用于共价连接与金黄色葡萄球菌蛋白A相连的蓖麻毒素。如此形成的复合物毒素通过内吞作用引入细胞质，能够杀死接种到动物体内的白血病细胞。免疫毒素与白血病细胞的相互作用在体外进行了一次、两次或三次处理，并在体内跟踪其对靶细胞的细胞毒性作用。免疫毒素处理之间的时间间隔通过对以单体形式包被特异性抗体、与蛋白A复合或与蛋白A-蓖麻毒素缀合物相互作用的EL4细胞进行膜周转研究来确定。三次处理后获得了99.8%的细胞杀伤率。
