Xu Yan-Long, Xu Xiu-Mei, Yang Zheng-Fei, Guo Mao-Juan, Jiang Xi-Juan
Department of Acupuncture and Moxibustion, Gansu Provincial Hospital of Traditional Chinese Medicine, Lanzhou 730050, China.
Department of Rehabilitation Medicine, Gansu Provincial Hospital of Traditional Chinese Medicine, Lanzhou 730050, China.
Zhen Ci Yan Jiu. 2020 Oct 25;45(10):789-92. doi: 10.13702/j.1000-0607.200076.
To observe the effect of electroacupuncture (EA) on the expression of soluble guanylate cyclase (sGC), cyclic guanosine phosphate (cGMP) and protein kinase G (PKG) of cerebral vascular smooth muscle in cerebral infarction (CI) rats, so as to study its dynamic regulation mechanism.
Male Wistar rats were randomly divided into normal control (=10), sham operation (=40), model (=40), and EA (=40) groups, and the latter three groups were further di-vided into 3, 6, 12 and 24 h subgroups (=10 in each subgroup). The CI model was established by occlusion of the middle cerebral artery (MCAO). EA(15 Hz, 2 mA)was applied to "Shuigou" (GV26) for 20 min. The cGMP, sGC and PKG activity and expression levels in the vascular smooth muscle of cerebral artery were detected using ELISA and Western blot, respectively.
After modeling, the immunoactivity and activities of sGC at 3 h, PKG at 3 and 6 h and cGMP from 3 h to 24 h were ob-viously decreased in the model group relevant to the normal control and sham-operation groups (<0.05, <0.01). After the intervention, the expression levels and activities of sGC at 3 h, PKG at 3 and 6 h and cGMP at 3 and 6 h were apparently up-regulated in the EA group relevant to the model group (<0.05).
EA intervention can significantly inhibit the down-regulation of sGC, PKG and cGMP expression of cerebral artery smooth muscle in MCAO model rats, which plays an important role in inhibiting cerebral artery smooth muscle spasm after ischemia, maintaining normal vascular function and state, and thus increasing blood perfusion around cerebral infarction area. However, acupuncture effect has a certain time-effectiveness.
观察电针（EA）对脑梗死（CI）大鼠脑血管平滑肌中可溶性鸟苷酸环化酶（sGC）、环磷酸鸟苷（cGMP）和蛋白激酶G（PKG）表达的影响，以探讨其动态调控机制。
雄性Wistar大鼠随机分为正常对照组（n = 10）、假手术组（n = 40）、模型组（n = 40）和电针组（n = 40），后三组再分为3、6、12和24 h亚组（每组n = 10）。采用大脑中动脉闭塞（MCAO）法制备CI模型。将电针（15 Hz，2 mA）施加于“水沟”（GV26）穴20 min。分别采用ELISA和Western blot法检测脑动脉血管平滑肌中cGMP、sGC和PKG的活性及表达水平。
造模后，模型组3 h时sGC的免疫活性和活性、3和6 h时PKG的活性以及3至24 h时cGMP的活性与正常对照组和假手术组相比明显降低（P < 0.05，P < 0.01）。干预后，电针组3 h时sGC的表达水平和活性、3和6 h时PKG的表达水平和活性以及3和6 h时cGMP的表达水平和活性与模型组相比明显上调（P < 0.05）。
电针干预可显著抑制MCAO模型大鼠脑动脉平滑肌sGC、PKG和cGMP表达的下调，对抑制缺血后脑动脉平滑肌痉挛、维持正常血管功能和状态、从而增加脑梗死灶周围血液灌注具有重要作用。然而，针刺效应具有一定的时效关系。
