Tomochika Shinobu, Kuwahara Taichi, Suzuki Nobuaki, Hazama Shoichi, Nagano Hiroaki
Dept. of Gastroenterological, Breast and Endocrine Surgery, Yamaguchi University Graduate School of Medicine.
Gan To Kagaku Ryoho. 2021 Mar;48(3):325-330.
Tumor infiltration of CD3 and CD8-positive T cells has been reported as a good prognostic marker for patients with colorectal cancer(CRC). To clarify the significance of CD4 and FOXP3-positive T cells in CRC for prognosis of intratumoral infiltration.
CD3, CD8, CD4 and FOXP3-positive T cells were immunostained(IHC)from tissue specimens of 342 CRC patients who underwent curative resection to quantify the number of infiltrating cells in the tumor. Microsatellite instability(MSI)was also evaluated in 322 samples and the clinicopathological factors and survival were analyzed.
Highly infiltrated groups of CD3, CD4 and FOXP3-positive T cells were associated with improved relapse-free survival(RFS). Highly infiltrated groups of CD8, CD4 and FOXP3-positive T cells were associated with improved disease- specific survival(DSS). Invasion depth, vascular infiltration, and CD4-positive T cell density were independent prognostic factors for DSS. CD4 and FOXP3-positive T cell infiltration was not associated with the high-frequency microsatellite instability group, in contrast to CD3 and CD8-positive T cell infiltration.
Intratumoral CD4-positive T cell density and FOXP3-positive T cell densities were stronger prognostic indicators than other clinicopathological features. These results may facilitate the establishment of novel prognostic factors and therapeutic strategies for CRC.
据报道，CD3和CD8阳性T细胞的肿瘤浸润是结直肠癌（CRC）患者的良好预后标志物。为阐明CRC中CD4和FOXP3阳性T细胞在肿瘤内浸润预后中的意义。
对342例行根治性切除的CRC患者的组织标本进行CD3、CD8、CD4和FOXP3阳性T细胞免疫染色（免疫组化），以量化肿瘤中浸润细胞的数量。还对322份样本进行了微卫星不稳定性（MSI）评估，并分析了临床病理因素和生存率。
CD3、CD4和FOXP3阳性T细胞高浸润组与无复发生存期（RFS）改善相关。CD8、CD4和FOXP3阳性T细胞高浸润组与疾病特异性生存期（DSS）改善相关。浸润深度、血管浸润和CD4阳性T细胞密度是DSS的独立预后因素。与CD3和CD8阳性T细胞浸润相反，CD4和FOXP3阳性T细胞浸润与高频微卫星不稳定性组无关。
肿瘤内CD4阳性T细胞密度和FOXP3阳性T细胞密度是比其他临床病理特征更强的预后指标。这些结果可能有助于建立CRC的新预后因素和治疗策略。
