Department of Ultrasound, Pudong New Area People's Hospital Affiliated to Shanghai University of Medicine & Health Sciences, Shanghai 201299, China.
Department of Radiology, Pudong New Area People's Hospital Affiliated to Shanghai University of Medicine & Health Sciences, Shanghai 201299, China.
Biomed Res Int. 2021 Mar 13;2021:6680356. doi: 10.1155/2021/6680356. eCollection 2021.
To detect the endothelial function of external iliac artery in rats with different stages of atherosclerosis by high-resolution ultrasound, so as to provide experimental methodological basis for evaluating the function of vascular endothelial cells by ultrasound.
The animals were randomly divided into the control group ( = 6) and the atherosclerosis model group ( = 15). The atherosclerosis group was further divided into 4-week group, 8-week group, and 12-week group, with 5 animals in each group. After separating and grinding rat spleen, the obtained cells were cultured by density gradient centrifugation. After the cells adhered, the morphology of the cells was observed under a microscope and identified by DiI-Ac-LDL and FITC-UEA-I double staining. The activities of LDH and SOD, the contents of MDA and GSH, and the contents of NO in plasma were detected by biochemical methods.
The protective effect of rosanilin on brain injury in rats with acute hypobaric hypoxia and its regulation on the expression of pAkt protein; ox-LDL inhibited the proliferation activity of EPCs in a concentration-dependent manner. The expression of KLF2 and S1PR1 in HAEC can be knocked down by small interfering RNA, and knocking down KLF2 can not only downregulate the expression of S1PR1 but also downregulate HAVEN. With the development of atherosclerosis, the endothelium-dependent relaxation function and endothelium-independent relaxation function of the control group and the atherosclerosis model at 4, 8, and 12 weeks were damaged in different degrees and gradually aggravated.
Atherosclerosis is a disease with both morphological and functional damage, and vascular endothelial function is damaged in the early stage with corresponding pathological changes. Ultrasound is an effective method to evaluate vascular endothelial function.
应用高分辨率超声检测不同阶段动脉粥样硬化大鼠髂外动脉内皮功能，为超声评价血管内皮细胞功能提供实验方法学基础。
动物随机分为对照组（n=6）和动脉粥样硬化模型组（n=15）。动脉粥样硬化组再分为 4 周组、8 周组和 12 周组，每组 5 只。分离研磨大鼠脾脏后，通过密度梯度离心对获得的细胞进行培养。细胞贴壁后，在显微镜下观察细胞形态，并用 DiI-Ac-LDL 和 FITC-UEA-I 双重染色进行鉴定。采用生化方法检测血浆中 LDH 和 SOD 的活性、MDA 和 GSH 的含量以及 NO 的含量。
Rosanilin 对急性低氧高气压脑损伤大鼠的保护作用及其对 pAkt 蛋白表达的调节作用；氧化型低密度脂蛋白（ox-LDL）呈浓度依赖性抑制 EPCs 的增殖活性。小干扰 RNA 可下调 HAEC 中 KLF2 和 S1PR1 的表达，敲低 KLF2 不仅下调 S1PR1 的表达，还下调 HAVEN。随着动脉粥样硬化的发展，对照组和 4、8、12 周动脉粥样硬化模型的内皮依赖性舒张功能和内皮非依赖性舒张功能均受到不同程度的损害，且逐渐加重。
动脉粥样硬化是一种既有形态又有功能损伤的疾病，血管内皮功能在早期就有相应的病理改变。超声是评价血管内皮功能的有效方法。
