Department of Laboratory Medicine, Weifang People's Hospital, Weifang, China.
Public Health Laboratory Centre, Kowloon, Hong Kong.
Am J Clin Pathol. 2021 Aug 4;156(3):445-454. doi: 10.1093/ajcp/aqaa260.
The present study was conducted to evaluate the usefulness of capillary electrophoresis (CE), cation-exchange high-performance liquid chromatography (HPLC), and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) for the screening of hemoglobin (Hb) variants prevalent in southern China.
A total of 102 types of Hb variants in 1,083 variant carriers were identified over a 5-year period. These variants were analyzed by a CE method (Capillarys 3 TERA), a cation-exchange HPLC analyzer (Variant II Turbo 2.0), and a MALDI-TOF MS system (QuanTOF).
The presence of 85 (83.3%, 85/102), 84 (82.4%, 84/102), and 62 (60.8%, 62/102) Hb variants was detected by Capillarys 3 TERA, Variant II Turbo 2.0, and QuanTOF, respectively. Of the three methods, only Capillarys 3 TERA recognized all 10 of the most frequent Hb variants in southern China. There were six, two, and three Hb variants that can only be detected by Capillarys 3 TERA, Variant II Turbo 2.0, and QuanTOF, respectively. The detection limit of mass difference for QuanTOF was approximately 11 to 20 Da.
MALDI-TOF MS is suitable for use as an auxiliary method rather than a stand-alone method for the screening of Hb variants prevalent in southern China.
本研究旨在评估毛细管电泳（CE）、阳离子交换高效液相色谱（HPLC）和基质辅助激光解吸/电离飞行时间（MALDI-TOF）质谱（MS）在筛查中国南方常见血红蛋白（Hb）变异体中的应用价值。
在 5 年期间，共鉴定了 1083 例变异携带者中的 102 种 Hb 变异体。这些变异体采用毛细管电泳法（Capillarys 3 TERA）、阳离子交换 HPLC 分析仪（Variant II Turbo 2.0）和 MALDI-TOF MS 系统（QuanTOF）进行分析。
Capillarys 3 TERA、Variant II Turbo 2.0 和 QuanTOF 分别检测到 85（83.3%，85/102）、84（82.4%，84/102）和 62（60.8%，62/102）种 Hb 变异体。在这三种方法中，只有 Capillarys 3 TERA 能识别中国南方最常见的 10 种 Hb 变异体。Capillarys 3 TERA 、Variant II Turbo 2.0 和 QuanTOF 分别可检测到 6、2 和 3 种 Hb 变异体。QuanTOF 的质量差异检测限约为 11 至 20 Da。
MALDI-TOF MS 适合作为筛查中国南方常见 Hb 变异体的辅助方法，而不是独立方法。
