Division of Clinical Veterinary Medicine, Ethics and Jurisprudence, Faculty of Veterinary Sciences and Animal Husbandry, Shuhama, Srinagar, Jammu and Kashmir, India.
Department of Veterinary Medicine, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India.
Trop Anim Health Prod. 2021 Mar 31;53(2):237. doi: 10.1007/s11250-021-02682-7.
Information on bio-type profile of Mycobacterium avium subspecies paratubeculosis (MAP) in sheep flocks and goat herds of Himalayan region is not reported earlier. The aim of our study was to determine the bio-type of MAP infecting livestock of this region. A total of 71 faecal samples (sheep-57, goats-14) were screened by Ziehl-Neelsen (ZN) staining and IS900 PCR, and then processed for culture on Herrold's egg yolk medium (HEYM) having mycobactin J (MJ). Out of 71 faecal samples, MAP colonies were seen only in four samples (sheep-3 and goat-1). Isolates were confirmed as MAP on the basis of slow growth, acid fastness, MJ dependency, IS900 and IS1311 PCR. All the IS900 and IS1311 PCR positive samples were bio-typed by IS1311 PCR-REA (restriction endonuclease analysis), which confirmed all four isolates as 'bison type.' In IS1311 based phylogeny of MAP isolates by ClustalW method of the MegAlign program of DNASTAR Lasergene software, the four sequences of MAP isolates (NCBI sequence nos. MH988763, MH988765, MH988766 and MH988764) did not show any distinct clustering/grouping pattern. However, these four isolates showed a bit of closeness to the MAP sequences (KC990353.1 and KC990352.1) of 'bison type' isolated from wood bison in Canada. In conclusion, this is the first report on isolation and bio-type profile of MAP infecting sheep and goats of Himalayan region. Study will help in devising prevention and control strategies against spread of MAP infection in livestock population of Himalayan region.
关于喜马拉雅地区绵羊和山羊群体中鸟分枝杆菌亚种副结核（MAP）的生物型谱信息以前没有报道过。我们的研究目的是确定该地区感染家畜的 MAP 生物型。共筛选了 71 份粪便样本（绵羊 57 份，山羊 14 份），采用齐-尼染色和 IS900 PCR 进行检测，然后在含有 Mycobactin J (MJ) 的 Herrold 鸡蛋黄培养基 (HEYM) 上进行培养。在 71 份粪便样本中，仅在 4 份样本（绵羊 3 份，山羊 1 份）中观察到 MAP 菌落。根据生长缓慢、耐酸、对 MJ 的依赖性、IS900 和 IS1311 PCR ，确认分离株为 MAP。所有 IS900 和 IS1311 PCR 阳性样本均通过 IS1311 PCR-REA（限制性内切酶分析）进行生物分型，结果证实所有 4 个分离株均为“野牛型”。在使用 DNASTAR Lasergene 软件的 MegAlign 程序的 ClustalW 方法对 MAP 分离株进行的基于 IS1311 的系统发育分析中，MAP 分离株的 4 个序列（NCBI 序列编号 MH988763、MH988765、MH988766 和 MH988764）没有显示出任何明显的聚类/分组模式。然而，这四个分离株与从加拿大木野牛中分离的“野牛型” MAP 序列（KC990353.1 和 KC990352.1）有一定的相似性。总之，这是首次报道喜马拉雅地区绵羊和山羊感染 MAP 的分离和生物型谱。该研究将有助于制定针对喜马拉雅地区家畜 MAP 感染传播的预防和控制策略。
