Chen Can, Zong Yi, Tang Jiaojiao, Ke Ruisheng, Lv Lizhi, Wu Mengchao, Lu Junhua
The Graduate School of Fujian Medical University, Fuzhou, Fujian, 350108, PR China.
Eastern Hepatobiliary Surgery Hospital, the Second Military Medical University, Shanghai, 200438, PR China.
Per Med. 2021 Jul;18(4):375-388. doi: 10.2217/pme-2020-0012. Epub 2021 Apr 1.
The aim of this study was to investigate the role of miR-369-3p in hepatocellular carcinoma (HCC). The expression levels of miR-369-3p were detected using the quantitative real-time reverse transcription-PCR analysis. The cell counting kit-8 and transwell assays were used to explore the effects of miR-369-3p on cell proliferation, migration and invasion of HCC cells. The miR-369-3p expression was downregulated in HCC tissues and cell lines, in comparison to the normal controls, respectively. , overexpression of miR-369-3p in Hep 3B and Huh7 cells inhibited cell proliferation, migration and invasion. SOX4 was a direct target of miR-369-3p. Our results suggested that miR-369-3p may be a tumor suppressor in HCC by targeting SOX4.
本研究旨在探讨miR-369-3p在肝细胞癌（HCC）中的作用。采用定量实时逆转录PCR分析检测miR-369-3p的表达水平。使用细胞计数试剂盒-8和Transwell实验来探究miR-369-3p对HCC细胞增殖、迁移和侵袭的影响。与正常对照相比，miR-369-3p在HCC组织和细胞系中的表达均下调。在Hep 3B和Huh7细胞中过表达miR-369-3p可抑制细胞增殖、迁移和侵袭。SOX4是miR-369-3p的直接靶点。我们的结果表明，miR-369-3p可能通过靶向SOX4在HCC中发挥肿瘤抑制作用。
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