硫酸铜对聚丙烯酰胺凝胶切片在过氧化氢中溶解的促进作用。

Enhancement of polyacrylamide gel slice dissolution in hydrogen peroxide by cupric sulfate.

作者信息

Donato H, Doig M T, Priest D G

机构信息

Department of Chemistry, College of Charleston, SC 29424.

出版信息

J Biochem Biophys Methods. 1988 Apr;15(6):331-5. doi: 10.1016/0165-022x(88)90086-3.

DOI:10.1016/0165-022x(88)90086-3

PMID:3379246

Abstract

An improved method is described for quantitation of radio-labelled protein by scintillation counting after polyacrylamide gel electrophoresis. The method is based upon copper catalyzed dissolution of gel slices in hydrogen peroxide under ambient conditions. Complete dissolution of gel sections was accomplished by incubation at 25 degrees C in 30% H2O2 that contained 0.9 mM CuSO4. Recovery of tritiated protein was greater than 90% under these conditions while in the absence of CuSO4 recovery was less than 50%.

摘要

描述了一种改进的方法，用于在聚丙烯酰胺凝胶电泳后通过闪烁计数对放射性标记蛋白质进行定量。该方法基于在环境条件下铜催化凝胶切片在过氧化氢中的溶解。通过在含有0.9 mM硫酸铜的30%过氧化氢中于25℃孵育，实现了凝胶切片的完全溶解。在这些条件下，氚标记蛋白质的回收率大于90%，而在没有硫酸铜的情况下，回收率小于50%。