基于茎环 qRT-PCR 的 miRNA 定量分析。

Stem-Loop qRT-PCR-Based Quantification of miRNAs.

机构信息

IGMM, CNRS, Université de Montpellier, Montpellier, France.

Université de Lorraine, CNRS, IMoPA, Nancy, France.

出版信息

Methods Mol Biol. 2021;2300:59-64. doi: 10.1007/978-1-0716-1386-3_6.

DOI:10.1007/978-1-0716-1386-3_6

PMID:33792871

Abstract

Stem-loop qRT-PCR is one of the most commonly used real-time PCR approach to quantify small non-coding RNAs such as microRNAs. The quantification method is divided in two steps. First, RNA is reverse transcribed using a specific stem-loop primer, and the resulting RT product is subsequently used as a template for quantitative real-time PCR. This fast and simple method provides quantitative data with high sensitivity and specificity to study miRNAs and their functions.

摘要

茎环 qRT-PCR 是最常用于定量分析小非编码 RNA（如 microRNAs）的实时 PCR 方法之一。该定量方法分为两个步骤。首先，使用特定的茎环引物反转录 RNA，然后将所得的 RT 产物用作定量实时 PCR 的模板。这种快速简单的方法提供了具有高灵敏度和特异性的定量数据，可用于研究 miRNA 及其功能。