Department of Orthopaedic Surgery, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, China.
Zhejiang Provincial Key Laboratory of Orthopaedics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, China.
Phytother Res. 2021 Jul;35(7):3848-3860. doi: 10.1002/ptr.7090. Epub 2021 Apr 1.
Random skin flap is widely used in plastic surgery. However, flap necrosis caused by ischemia-reperfusion injury limits its clinical applications. Apigenin, a naturally occurring flavonoid mainly derived from plants, facilitates flap survival. In this study, we explored the effects of apigenin on flap survival and the underlying mechanisms. A total of 54 mice having a dorsal random flap model were randomly divided into control, apigenin, and apigenin +3-methyladenine groups. These groups were treated with dimethyl sulfoxide solution, apigenin, and apigenin +3-methyladenine, respectively. The animals were then euthanized to assess angiogenesis, apoptosis, oxidative stress, and autophagy levels through histological and protein analyses. Apigenin promotes survival of the skin flap area and reduces tissue edema. In addition, apigenin enhanced angiogenesis, attenuated apoptosis, alleviated oxidative stress, and activated autophagy. Interestingly, 3-methyladenine reversed the effects of apigenin on flap survival, angiogenesis, apoptosis, and oxidative stress through inhibition of autophagy. The findings of this study show that apigenin promotes angiogenesis, inhibits cell apoptosis, and lowers oxidative stress by mediating autophagy, thus the improving survival rate of random skin flaps.
随机皮瓣被广泛应用于整形手术。然而，由于缺血再灌注损伤导致的皮瓣坏死限制了其临床应用。芹菜素是一种天然存在的类黄酮，主要来源于植物，它可以促进皮瓣存活。在这项研究中，我们探讨了芹菜素对皮瓣存活的影响及其潜在机制。总共 54 只小鼠建立了背部随意皮瓣模型，随机分为对照组、芹菜素组和芹菜素+3-甲基腺嘌呤组。这些组分别用二甲基亚砜溶液、芹菜素和芹菜素+3-甲基腺嘌呤处理。然后处死动物，通过组织学和蛋白质分析评估血管生成、细胞凋亡、氧化应激和自噬水平。芹菜素促进皮瓣区域的存活并减少组织水肿。此外，芹菜素通过促进血管生成、抑制细胞凋亡、减轻氧化应激和激活自噬来改善皮瓣的存活。有趣的是，3-甲基腺嘌呤通过抑制自噬逆转了芹菜素对皮瓣存活、血管生成、细胞凋亡和氧化应激的影响。本研究结果表明，芹菜素通过调节自噬来促进血管生成、抑制细胞凋亡和降低氧化应激，从而提高随意皮瓣的存活率。
