大鼠肠道中的固醇载体蛋白2样活性。
Sterol carrier protein2-like activity in rat intestine.
作者信息
Kharroubi A, Wadsworth J A, Chanderbhan R, Wiesenfeld P, Noland B, Scallen T, Vahouny G V, Gallo L L
机构信息
Department of Biochemistry, George Washington University School of Medicine and Health Sciences, Washington, DC 20037.
出版信息
J Lipid Res. 1988 Mar;29(3):287-92.
A sterol carrier protein2 (SCP2)-like activity has been demonstrated in rat intestinal mucosal homogenates and in isolated intestinal cells from both crypt and villus zones. The results indicate the presence of a protein with similar molecular weight and antigenicity to that of authentic SCP2 purified from rat liver cytosol. Like liver SCP2, mucosal cytosol stimulates pregnenolone production in rat adrenal mitochondria and acyl coenzyme A:cholesterol acyltransferase activity of liver and mucosal microsomes. The distribution of SCP2-like activity as determined by radioimmunoassay indicates high levels in mitochondria and cytosol and relatively lower levels in microsomes and in brush-border membranes. The widespread distribution of SCP2-like protein in the intestine is consistent with potential transfer functions in all phases of cholesterol processing.
在大鼠肠黏膜匀浆以及来自隐窝和绒毛区的分离肠细胞中，已证实存在一种类似固醇载体蛋白2（SCP2）的活性。结果表明，存在一种蛋白质，其分子量和抗原性与从大鼠肝细胞溶质中纯化的 authentic SCP2 相似。与肝脏 SCP2 一样，黏膜细胞溶质可刺激大鼠肾上腺线粒体中孕烯醇酮的生成以及肝脏和黏膜微粒体的酰基辅酶 A：胆固醇酰基转移酶活性。通过放射免疫测定法确定的 SCP2 样活性分布表明，线粒体和细胞溶质中的水平较高，而微粒体和刷状缘膜中的水平相对较低。SCP2 样蛋白在肠道中的广泛分布与胆固醇加工所有阶段的潜在转运功能一致。
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