Pharmacology and Toxicology Department, School of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.
J Pharm Pharmacol. 2021 Oct 7;73(11):1539-1546. doi: 10.1093/jpp/rgaa066.
The effects of Crocin as a cardioprotective material against Aluminum phosphide poisoning by reducing the oxidative stress is investigated.
The level of biomarkers of oxidative stress (Catalase, Superoxide dismutase, Malondialdehyde and Protein carbonyl) were measured in the cell culture model on Human Cardiac Myocyte cells to detect the protective effect of crocin. Initially, to define the pure impact of aluminum phosphide poison and crocin on the heart cells, their effects on the biomarkers quantity in cell line were measured, separately, using the standard related kits. Later the effect of crocin with different concentration as a treatment on the oxidative stress biomarkers of the poisoned heart cells were monitored. Note that in pre-treatment case, the crocin was initially added to the cells before poisoning them. Data were analyzed using the analysis of variance method.
Results showed that crocin treatment reduced the aluminum phosphide (AlP) poisoning effect significantly. The treatment resulted in substantial deviation in the biomarkers of oxidative stress at the pre- and post-treatment phases for all groups. The oxidative markers values of the poisoned cells were recovered by crocin treatment.
Crocin is proposed as a potentially powerful antioxidant to treat the cardiotoxicity caused by aluminum phosphide poisoning.
研究西红花酸作为一种心脏保护物质，通过减少氧化应激，对磷化铝中毒的保护作用。
在人心脏肌细胞的细胞培养模型中，测量氧化应激生物标志物（过氧化氢酶、超氧化物歧化酶、丙二醛和蛋白质羰基）的水平，以检测西红花酸的保护作用。首先，为了确定磷化铝中毒和西红花酸对心脏细胞的纯影响，使用标准相关试剂盒分别测量它们对细胞系中生物标志物数量的影响。然后，监测不同浓度的西红花酸作为治疗药物对中毒心脏细胞氧化应激生物标志物的影响。需要注意的是，在预处理情况下，西红花酸在细胞中毒之前先加入细胞中。数据采用方差分析方法进行分析。
结果表明，西红花酸处理显著降低了磷化铝（AlP）的中毒效应。治疗组在预处理和后处理阶段，所有组的氧化应激生物标志物都有显著差异。西红花酸处理恢复了中毒细胞的氧化标记物值。
西红花酸可作为一种潜在的强大抗氧化剂，用于治疗磷化铝中毒引起的心脏毒性。
