Key Laboratory for Palygorskite Science and Applied Technology of Jiangsu Province, Huaiyin Institute of Technology, Huai'an 223003, PR China; Guangzhou University of Chinese Medicine, National Engineering Research Center for Modernization of Traditional Chinese Medicine, Guangzhou 510006, PR China.
Department of Imaging, Affiliated Huai'an Hospital of Xuzhou Medical University, Huai'an 223001, Jiangsu, PR China.
Int J Biol Macromol. 2021 Jul 1;182:343-353. doi: 10.1016/j.ijbiomac.2021.03.163. Epub 2021 Mar 29.
A large amount of protein impurity severely restricts the application of polysaccharides of Ruditapes philippinarum (PRP) in food and medicine. Moreover, the traditional Sevag deproteinization method always involves organic reagents. The purpose of this paper was to develop an effective, green and mild deproteinization method from PRP by attapulgite-based silk fibroin composite aerogel (ASA). Firstly, ASA was synthesized and applied to remove protein from PRP. Secondly, the deproteinization parameters were optimized with selectivity coefficient as index as follows: dose of ASA 1% and pH 7.0. Under these conditions, deproteinization ratio (Dr%), polysaccharide recovery ratio (Rr%) and selectivity coefficient (Kc) reached 79.44 ± 1.87%, 95.81 ± 2.95% and 18.95 ± 1.55, respectively. Next, the feasibility of ASA method was evaluated. As a result, ASA method not only achieved higher deproteinization efficiency in less time compared with Sevag method, but also retained structure and antioxidant activity of polysaccharides. ASA was also proven with recycling ability and could be reused more than five times. Furthermore, it was found that protein adsorption on ASA was better fitted by pseudo second-order kinetic and Freundlich model. Taking together, the data implied that ASA method would be promising of deproteinization from PRP suitable for polysaccharides processing.
大量的蛋白质杂质严重限制了菲律宾蛤仔多糖（PRP）在食品和医药中的应用。此外，传统的 Sevag 脱蛋白法通常涉及有机溶剂。本文旨在通过基于凹凸棒土的丝素蛋白复合气凝胶（ASA）从 PRP 中开发一种有效、绿色和温和的脱蛋白方法。首先，合成了 ASA 并将其应用于从 PRP 中去除蛋白质。其次，以选择性系数为指标，优化了脱蛋白参数，如下：ASA 用量为 1%，pH 值为 7.0。在此条件下，脱蛋白率（Dr%）、多糖回收率（Rr%）和选择性系数（Kc）分别达到 79.44±1.87%、95.81±2.95%和 18.95±1.55%。接下来，评估了 ASA 方法的可行性。结果表明，与 Sevag 法相比，ASA 法不仅在更短的时间内达到了更高的脱蛋白效率，而且还保留了多糖的结构和抗氧化活性。ASA 还被证明具有回收能力，可重复使用超过五次。此外，还发现蛋白质在 ASA 上的吸附更符合拟二级动力学和 Freundlich 模型。综上所述，数据表明 ASA 法有望成为从 PRP 中脱蛋白的一种方法，适用于多糖加工。
