循环滤泡辅助性 T 细胞及其亚群分布与乙型肝炎疫苗低应答相关。

Circulating Tfh cell and subsets distribution are associated with low-responsiveness to hepatitis B vaccination.

机构信息

Department of Preventive Medicine, Guangdong Medical University, Dongguan, China.

School Clinic, Guangdong Medical University, Dongguan, China.

出版信息

Mol Med. 2021 Apr 1;27(1):32. doi: 10.1186/s10020-021-00290-7.

BACKGROUND

An estimated 5-10 % of healthy vaccinees lack adequate antibody response following receipt of a standard three-dose hepatitis B vaccination regimen. The cellular mechanisms responsible for poor immunological responses to hepatitis B vaccine have not been fully elucidated to date.

METHODS

There were 61 low responders and 56 hyper responders involved in our study. Peripheral blood samples were mainly collected at D7, D14 and D28 after revaccinated with a further dose of 20 µg of recombinant hepatitis B vaccine.

RESULTS

We found low responders to the hepatitis B vaccine presented lower frequencies of circulating follicular helper T (cTfh) cells, plasmablasts and a profound skewing away from cTfh2 and cTfh17 cells both toward cTfh1 cells. Importantly, the skewing of Tfh cell subsets correlated with IL-21 and protective antibody titers. Based on the key role of microRNAs involved in Tfh cell differentiation, we revealed miR-19b-1 and miR-92a-1 correlated with the cTfh cell subsets distribution and antibody production.

CONCLUSIONS

Our findings highlighted a decrease in cTfh cells and specific subset skewing contribute to reduced antibody responses in low responders.

背景

据估计，在接受标准的三剂乙型肝炎疫苗接种方案后，约有 5-10%的健康疫苗接种者缺乏足够的抗体反应。迄今为止，尚未完全阐明导致乙型肝炎疫苗免疫反应不良的细胞机制。

方法

我们的研究纳入了 61 名低应答者和 56 名高应答者。在接种 20μg 重组乙型肝炎疫苗加强针后，于 D7、D14 和 D28 采集外周血样本。

结果

我们发现乙型肝炎疫苗低应答者循环滤泡辅助 T（cTfh）细胞、浆母细胞频率较低，cTfh2 和 cTfh17 细胞向 cTfh1 细胞显著倾斜。重要的是，Tfh 细胞亚群的倾斜与 IL-21 和保护性抗体滴度相关。基于 microRNAs 在 Tfh 细胞分化中的关键作用，我们揭示了 miR-19b-1 和 miR-92a-1 与 cTfh 细胞亚群分布和抗体产生相关。