Department of Food Science and Technology, School of Agriculture, Shiraz University, Shiraz, Iran.
Seafood Processing Research Group, School of Agriculture, Shiraz University, Shiraz, Iran.
Sci Rep. 2021 Apr 1;11(1):7401. doi: 10.1038/s41598-021-86808-9.
The multifunctional properties of fish gelatin hydrolysates have not been completely elucidated. Here, the biological characterization of these peptides was performed to engineer multifunctional peptides. Bioactive peptides were produced from mackerel byproducts via successive enzymatic hydrolysis reactions using subtilisin A and actinidin as microbial and herbal proteases. The antibacterial activity against both gram-negative and -positive food-borne pathogens, including Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Klebsiella pneumoniae, as well as the inhibitory potential of angiotensin-converting enzyme (ACE) and dipeptidyl peptidase IV (DPP-IV), was accessed in vitro. The synthesized peptides demonstrated multifunctional properties, which were further confirmed by in silico protocols. The ACE and DPP-IV inhibitory (IC) values of P1, P2, and P3 were 0.92 and 0.87, 0.51 and 0.93, 0.78 and 1.16 mg mL, respectively. Moreover, the binding energy was sufficient for all three peptides to inhibit both ACE and DPP-IV enzymes with excellent three-dimensional conformation (RMSD = 0.000) for all six docking mechanisms.
鱼明胶水解物的多功能特性尚未完全阐明。在这里，通过使用枯草杆菌蛋白酶 A 和菠萝蛋白酶作为微生物和植物蛋白酶对这些肽进行连续的酶解反应，对这些肽的生物学特性进行了研究，以构建多功能肽。通过连续的酶解反应，从鲭鱼副产物中生成了具有生物活性的肽，所用的酶为枯草杆菌蛋白酶 A 和菠萝蛋白酶。通过体外实验评估了这些肽对革兰氏阴性和革兰氏阳性食源性病原体（包括大肠杆菌、铜绿假单胞菌、金黄色葡萄球菌和肺炎克雷伯菌）的抗菌活性，以及它们对血管紧张素转换酶（ACE）和二肽基肽酶 IV（DPP-IV）的抑制潜力。所合成的肽具有多功能特性，这一点通过计算机模拟协议得到了进一步的证实。P1、P2 和 P3 的 ACE 和 DPP-IV 抑制（IC）值分别为 0.92 和 0.87、0.51 和 0.93、0.78 和 1.16 mg mL。此外，所有三种肽都具有足够的结合能，能够抑制 ACE 和 DPP-IV 两种酶，所有六种对接机制的三维构象都非常好（RMSD = 0.000）。
