Yasukuni Ryohei, Minamino Daiki, Iino Takanori, Araki Takashi, Takao Kohei, Yamada Sohei, Bessho Yasumasa, Matsui Takaaki, Hosokawa Yoichiroh
Division of Materials Science, Graduate School of Science and Technology, Nara Institute of Science and Technology, Ikoma, Nara 630-0192, Japan.
Division of Biological Science, Graduate School of Science and Technology, Nara Institute of Science and Technology, Ikoma, Nara 630-0192, Japan.
Biomed Opt Express. 2021 Feb 9;12(3):1366-1374. doi: 10.1364/BOE.414338. eCollection 2021 Mar 1.
Mechanical properties of cells and tissues closely link to their architectures and physiological functions. To obtain the mechanical information of submillimeter scale small biological objects, we recently focused on the object vibration responses when excited by a femtosecond laser-induced impulsive force. These responses are monitored by the motion of an AFM cantilever placed on top of a sample. In this paper, we examined the surface cellular stiffness of zebrafish embryos based on excited vibration forms in different cytoskeletal states. The vibration responses were more sensitive to their surface cellular stiffness in comparison to the Young's modulus obtained by a conventional AFM force curve measurement.
细胞和组织的力学特性与其结构和生理功能密切相关。为了获取亚毫米尺度小生物物体的力学信息，我们最近聚焦于飞秒激光诱导脉冲力激发时物体的振动响应。这些响应通过放置在样品顶部的原子力显微镜（AFM）悬臂的运动来监测。在本文中，我们基于不同细胞骨架状态下的激发振动形式，研究了斑马鱼胚胎的表面细胞硬度。与通过传统AFM力曲线测量获得的杨氏模量相比，振动响应对其表面细胞硬度更为敏感。
