Department of Biophysics, Faculty of Medicine, Mersin University, Mersin, Turkey.
Department of Biostatistics and Medical Informatics, Faculty of Medicine, Mersin University, Mersin, Türkiye.
Electromagn Biol Med. 2021 Jul 3;40(3):408-419. doi: 10.1080/15368378.2021.1907403. Epub 2021 Apr 2.
Some experimental research indicates that low-frequency pulsed electromagnetic field (PEMF) stimulation may accelerate regeneration in sciatic nerve injury. However, little research has examined the electrophysiological and functional properties of regenerating peripheral nerves under PEMF. The main aim of the present study is to investigate the effects of PEMF on sciatic nerve regeneration in short- and long-term processes with electrophysiologically and functionally after crushing damage. Crush lesions were performed using jewelery forceps for 30 s. After crush injury of the sciatic nerves, 24 female Wistar-Albino rats were divided into 3 groups with 8 rats in each group: SH(Sham), SNI (Sciatic Nerve Injury), SNI+PEMF(Sciatic Nerve Injury+Pulsed Electromagnetic Field). SNI+PEMF group was exposed to PEMF (4 h/day, intensity; 0.3mT, low-frequency; 2 Hz) for 40-days. Electrophysiological records (at the beginning and 1st, 2nd, 4th and 6th weeks post-crush) and functional footprints (at 1st, 2nd, 3rd, 4th, 5th and 6th weeks post crush) were measured from all groups during the experiment. The results were compared to SNI and SNI+PEMF groups, it was found that amplitude and area parameters in the first-week were significantly higher and latency was lower in the SNI+PEMF group than in the SNI group ( < 0,05). However, the effect of PEMF was not significant in the 2nd, 4th, 6th weeks. In addition, in the 1st and 2nd weeks, the SSI parameters were significantly higher in SNI+PMF group than SNI group ( < .05). These results indicate that low-frequency PEMF is not effective for long-periods of application time while PEMF may be useful during the short-term recovery period.
一些实验研究表明，低频脉冲电磁场（PEMF）刺激可能加速坐骨神经损伤的再生。然而，很少有研究检查 PEMF 下再生周围神经的电生理和功能特性。本研究的主要目的是研究 PEMF 在短期和长期过程中对挤压损伤后坐骨神经再生的影响，从电生理和功能上进行研究。使用珠宝镊子进行挤压损伤 30 秒。坐骨神经损伤后，24 只雌性 Wistar-Albino 大鼠分为 3 组，每组 8 只：SH（假手术）、SNI（坐骨神经损伤）、SNI+PEMF（坐骨神经损伤+脉冲电磁场）。SNI+PEMF 组每天暴露于 PEMF（4 小时，强度为 0.3mT，低频为 2Hz）40 天。在实验过程中，从所有组中测量电生理记录（损伤后第 1、2、4 和 6 周开始）和功能足迹（损伤后第 1、2、3、4、5 和 6 周）。与 SNI 和 SNI+PEMF 组相比，结果发现，第 1 周的振幅和面积参数显著较高，潜伏期较低，SNI+PEMF 组明显低于 SNI 组（ < 0.05）。然而，PEMF 的作用在第 2、4、6 周并不显著。此外，在第 1 和第 2 周，SNI+PMF 组的 SSI 参数明显高于 SNI 组（ < 0.05）。这些结果表明，低频 PEMF 在长时间应用时无效，而 PEMF 在短期恢复期可能有用。
