Corsinovi Debora, Usai Alice, Sarlo Miriam De, Giannaccini Martina, Ori Michela
Department of Translational Research and of New Surgical and Medical Technologies, University of Pisa, Pisa, Italy.
Department of Biology, University of Pisa, Pisa, Italy.
Anticancer Agents Med Chem. 2022;22(4):748-759. doi: 10.2174/1871520621666210402111634.
Zebrafish (Danio rerio) is a vertebrate that has become a popular alternative model for the cellular and molecular study of human tumors and for drug testing and validating approaches. Notably, zebrafish embryos, thanks to their accessibility, allow rapid collection of in vivo results prodromal to validation in the murine models in respect to the 3R principles. The generation of tumor xenograft in zebrafish embryos and larvae, or zebrafish avatar, represents a unique opportunity to study tumor growth, angiogenesis, cell invasion and metastatic dissemination, interaction between tumor and host in vivo avoiding immunogenic rejection, representing a promising platform for the translational research and personalized therapies.
In this mini-review, we report recent advances in breast cancer research and drug testing that took advantage of the zebrafish xenograft model using both breast cancer cell lines and patient's biopsy.
Patient derived xenograft, together with the gene editing, the omics biotechnology, the in vivo time lapse imaging and the high-throughput screening that are already set up and largely used in zebrafish, could represent a step forward towards precision and personalized medicine in the breast cancer research field.
斑马鱼（Danio rerio）是一种脊椎动物，已成为人类肿瘤细胞和分子研究以及药物测试与验证方法的热门替代模型。值得注意的是，斑马鱼胚胎因其易获取性，能够依据3R原则在小鼠模型验证之前快速收集体内实验结果。在斑马鱼胚胎和幼体中生成肿瘤异种移植，即斑马鱼替身，为研究肿瘤生长、血管生成、细胞侵袭和转移扩散、肿瘤与宿主在体内的相互作用且避免免疫排斥反应提供了独特机会，是转化研究和个性化治疗的一个有前景的平台。
在本综述中，我们报告了利用斑马鱼异种移植模型进行乳腺癌研究和药物测试的最新进展，该模型使用了乳腺癌细胞系和患者活检样本。
患者来源的异种移植，连同已在斑马鱼中建立并广泛应用的基因编辑、组学生物技术、体内延时成像和高通量筛选，可能代表着乳腺癌研究领域向精准和个性化医学迈进的一步。
