Division of Veterinary Parasitology, Faculty of Veterinary Sciences, SKUAST-Jammu, R S Pura, Jammu, India.
Division of Veterinary Pathology, Faculty of Veterinary Sciences, SKUAST-Kashmir, Shuhama, Srinagar, India.
Trop Biomed. 2021 Mar 1;38(1):86-93. doi: 10.47665/tb.38.1.015.
The study was aimed to evaluate the effectiveness of maggot therapy in healing of cutaneous infected wound in streptozotocin (STZ) induced diabetic Wistar rat. For live maggots, the sterilized eggs of Lucilia sericata were obtained from colonies established in laboratory. Diabetes model was established in 48 male Wister rat by intra-peritoneal injection of STZ at the dose of 60 mg/kg body-weight. Cutaneous wounds exposed with mixed colonies of bacteria like Staphylococcus aureus, E. coli and Pseudomonas aeruginosa were prepared in all rat. The animals equally divided in 4 groups with 12 rats each being presented as treatment group of control, antibiotic, maggot and maggot with antibiotic in combination. All treatments were done once and hold for 24 hours. Wound kinetics and bacterial bio burden were measured at weekly interval to till complete healing. Significant reduction in wound area with maximum contraction was found (>95%) in maggot treated group when compared to antibiotic treated (79%) and control (72%). In maggot as well as maggot and antibiotic in combination group showed early elimination of bacterial bio-burden 7.88±0.03log CFU/ml to 1.12±0.65log CFU/ml and 7.86±0.04) log CFU/ml to 1.54±0.52log CFU/ml respectively in three weeks of time. Early healing indication was also experienced on histomorphological examination of wounded tissue of maggot treated groups by early and better epithelialization, collagenation and neovascularization with complete healing of wound in three weeks in comparison to antibiotic and control respectively. However, the present study did not show any difference in healing of wound with use of maggot alone or in antibiotic combination. Live maggot of Lucilia sericata effectively lower bacterial bioburden and and accelerate healing of infected cutaneous wound in diabetic conditions.
该研究旨在评估蛆虫疗法在治疗链脲佐菌素（STZ）诱导的糖尿病 Wistar 大鼠皮肤感染性伤口中的疗效。对于活蛆，从实验室建立的殖民地中获得了丝光绿蝇的无菌卵。通过腹腔注射 STZ（剂量为 60mg/kg 体重）在 48 只雄性 Wister 大鼠中建立糖尿病模型。在所有大鼠中制备了暴露于金黄色葡萄球菌、大肠杆菌和铜绿假单胞菌等混合细菌的皮肤伤口。动物分为 4 组，每组 12 只，分别为对照组、抗生素组、蛆虫组和蛆虫联合抗生素组。所有治疗均进行一次，持续 24 小时。每周测量一次伤口动力学和细菌生物负荷，直到完全愈合。与抗生素治疗组（79%）和对照组（72%）相比，蛆虫治疗组的伤口面积显著减少，最大收缩率（>95%）。在蛆虫组以及蛆虫和抗生素联合组中，细菌生物负荷在 3 周内从 7.88±0.03log CFU/ml 减少到 1.12±0.65log CFU/ml 和 7.86±0.04)log CFU/ml 分别减少到 1.54±0.52log CFU/ml。在 3 周的时间内，蛆虫治疗组的组织学形态学检查也显示出早期愈合迹象，通过早期和更好的上皮化、胶原化和新生血管化，与抗生素和对照组相比，伤口更快愈合。然而，本研究没有显示单独使用蛆虫或与抗生素联合使用在伤口愈合方面有任何差异。丝光绿蝇的活蛆可有效降低细菌生物负荷，加速糖尿病条件下感染性皮肤伤口的愈合。
