State Key Laboratory of Tropical Oceanography, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, China.
Daya Bay Marine Biology Research Station, Chinese Academy of Sciences, Shenzhen, 518121, China.
Ecotoxicology. 2021 Jul;30(5):929-935. doi: 10.1007/s10646-021-02379-5. Epub 2021 Apr 2.
The pollution of polycyclic aromatic hydrocarbons was serious in sediments of the Pearl River estuary, China. A fluorene-degrading bacterium, strain A2-3, was isolated from hydrocarbon contaminated sediment of this estuary and identified as Rhodococcus sp. based on the analyses of 16S rRNA gene sequence and morphology. Rhodococcus sp. A2-3 can take naphthalene, p-Teropheny, fluorene, pyrene, salicylic acid, citric acid, acetic acid, diethyletheranhydrous, methanol or 4,4'-dibromodiphenyl ether as sole carbon source. 100% of 100 mg/L fluorene or 89% of 400 mg/L fluorene was removed in 7 days by strain A2-3 at 30 °C and pH 7.5. The strain A2-3 showed a high degradation efficiency of fluorene when pH values ranged from 5.5 to 8.5. The proposed pathway of fluorene catabolism by strain A2-3 was initially attacked by 3,4 dioxygenation. Our results suggested Rhodococcus sp. A2-3 can degrade PAHs under aerobic conditions and can function in bioremediation, particularly for weakly acid environment.
珠江口沉积物中多环芳烃污染严重。从该河口烃类污染沉积物中分离到一株菲降解菌，A2-3 菌株，根据 16S rRNA 基因序列和形态分析，鉴定为 Rhodococcus sp.。Rhodococcus sp. A2-3 可以利用萘、对甲苯、芴、芘、水杨酸、柠檬酸、乙酸、无水二乙醚、甲醇或 4,4'-二溴二苯醚作为唯一的碳源。在 30°C 和 pH 7.5 下，A2-3 菌株在 7 天内可去除 100mg/L 芴的 100%或 400mg/L 芴的 89%。当 pH 值在 5.5 到 8.5 之间时，A2-3 菌株对芴的降解效率很高。A2-3 菌株对芴的代谢途径最初是由 3,4 加氧作用攻击的。我们的结果表明，Rhodococcus sp. A2-3 可以在好氧条件下降解 PAHs，并可用于生物修复，特别是在弱酸性环境中。
