[电针对神经病理性疼痛大鼠脊髓中血管生成素-1的影响]
[Effect of electroacupuncture on angiopoietin-1 in spinal cord of rats with neuropathic pain].
作者信息
Wu Qiao-Yun, Zhou Ke-Cheng, Yue Jing-Jing, Tu Wen-Zhan, Yang Guan-Hu, Jiang Song-He
机构信息
Department of Physical Medicine and Rehabilitation, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou 325027, Zhejiang Province, China; Integrative & Optimized Medicine Research Center, China-USA Institute for Acupuncture and Rehabilitation, Wenzhou Medical University, Wenzhou 325027, Zhejiang Province.
Integrative & Optimized Medicine Research Center, China-USA Institute for Acupuncture and Rehabilitation, Wenzhou Medical University, Wenzhou 325027, Zhejiang Province.
出版信息
Zhen Ci Yan Jiu. 2021 Mar 25;46(3):209-14. doi: 10.13702/j.1000-0607.200269.
OBJECTIVE
To observe the effect of electroacupuncture (EA) on the behavior, histomorphology and the expression of angiopoietin-1 (Angpt-1) in rats with spinal nerve injury, so as to explore its mechanism on neuropathic pain.
METHODS
Forty-five male SD rats were randomly divided into sham, model and EA groups (=15 rats in each group). Spinal nerve ligation (SNL) of the L5 lumbar vertebra was performed to establish a rat model of neuropathic pain. The rats in the EA group were given EA at "Zusanli" (ST36) and "Kunlun" (BL60) of the operation side with continuous wave at a frequency of 2 Hz and an intensity of 1.5 mA once a day, 30 minutes each time for 7 days. The sham group only exposed L5 spinal nerves without ligation. Mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) were observed and recorded before modeling and on days 3,5,7,10,12 and 14 after modeling. L4-L6 segments of spinal cord were taken and the morphological changes of spinal dorsal horn were observed by HE staining. The changes of spinal dorsal horn nerve fiber structure were observed by silver plating staining. Angpt-1 expression was detected by Western blot and immunohistochemistry.
RESULTS
Compared with the sham group, the model group had significant reductions in MWT and TWL at each time point (<0.01); compared with the model group, the EA group had significant increases in MWT and TWL on days 10,12 and 14 after intervention (<0.05, <0.01). HE staining showed that in the model group, the spinal dorsal horn showed degeneration and necrosis of neurons, nuclear fixation and shrinkage, and loose surrounding tissues. The degree of tissue damage of the EA group was milder than that of the model group. The silver staining results showed the model group had obvious neuronal fibrillary tangles, while there were fewer neuronal fibrillary tangles in the EA group. Compared with the sham group, the Angpt-1 expression in the model group was significantly decreased (<0.01), and compared with the model group, the EA group had a significant increase in the expression of Angpt-1 (<0.01).
CONCLUSION
EA can promote the recovery of nerve function in SNL rats by up-regulating Angpt-1 expression.
目的
观察电针对脊髓神经损伤大鼠行为、组织形态学及血管生成素-1（Angpt-1）表达的影响，以探讨其治疗神经病理性疼痛的机制。
方法
将45只雄性SD大鼠随机分为假手术组、模型组和电针组，每组15只。采用L5腰椎脊髓神经结扎（SNL）法建立神经病理性疼痛大鼠模型。电针组大鼠于手术侧“足三里”（ST36）和“昆仑”（BL60）穴给予电针治疗，连续波，频率2Hz，强度1.5mA，每日1次，每次30分钟，共7天。假手术组仅暴露L5脊髓神经，不进行结扎。分别于造模前及造模后第3、5、7、10、12和14天观察并记录机械缩足阈值（MWT）和热缩足潜伏期（TWL）。取脊髓L4-L6节段，行HE染色观察脊髓背角形态学变化，行镀银染色观察脊髓背角神经纤维结构变化。采用蛋白质免疫印迹法和免疫组织化学法检测Angpt-1表达。
结果
与假手术组比较，模型组各时间点MWT和TWL均显著降低（<0.01）；与模型组比较，电针组干预后第10、12和14天MWT和TWL均显著升高（<0.05，<0.01）。HE染色显示，模型组脊髓背角神经元出现变性坏死，核固缩、核仁缩小，周围组织疏松；电针组组织损伤程度较模型组轻。镀银染色结果显示，模型组可见明显的神经元纤维缠结，电针组神经元纤维缠结较少。与假手术组比较，模型组Angpt-1表达显著降低（<0.01）；与模型组比较，电针组Angpt-1表达显著升高（<0.01）。
结论
电针可通过上调Angpt-1表达促进SNL大鼠神经功能恢复。
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