Sinanović O, Chiba S
Department of Pharmacology, Shinshu University School of Medicine, Matsumoto, Japan.
Jpn J Pharmacol. 1988 Mar;46(3):237-46. doi: 10.1254/jjp.46.237.
The stainless steel cannula inserting method was used to observe effects of alpha-adrenoceptor agonists, 5-HT and KCl before and after cold storage (3-5 days, at 4 degrees C) in skeletal muscle branches of the simian deep femoral artery. Epinephrine (EPI), norepinephrine (NE), phenylephrine (PE), methoxamine (MT) and 5-hydroxytryptamine (5-HT) induced marked monophasic vasoconstrictions in a dose-dependent manner. 5-HT induced a greater vasoconstriction in larger diameter vessels (old animals) than that in smaller ones (young animals), suggesting age-related responses. A selective alpha 2-stimulant, clonidine (CLO) or xylazine (XYL), produced only a slight vasoconstriction. Tyramine (TYR) also produced only a slight vasoconstrictor response. The order of potencies for inducing vasoconstrictions was EPI greater than or equal to 5-HT greater than or equal to NE greater than MT = PE much greater than KCl greater than CLO = XYL = TYR. The vasoconstrictor responses to all used adrenergic agonists and 5-HT were not significantly influenced by the prolonged cold storage. However, KCI-induced constrictions were significantly suppressed by the cold storage. These results suggest that the postjunctional alpha-adrenoceptor in simian skeletal muscle arteries is mainly of the alpha 1-type. Since cold storage caused a significant suppression of the KCl-induced response but not those of adrenoceptor agonists and 5-HT, it was considered that the mechanism of calcium entry to the vascular smooth muscle of skeletal muscle arteries might be significantly damaged by the cold storage.
采用不锈钢套管插入法，观察α-肾上腺素能受体激动剂、5-羟色胺（5-HT）和氯化钾在猴股深动脉骨骼肌分支冷保存（4℃，3 - 5天）前后的作用。肾上腺素（EPI）、去甲肾上腺素（NE）、去氧肾上腺素（PE）、甲氧明（MT）和5-羟色胺（5-HT）均以剂量依赖方式引起明显的单相血管收缩。5-HT在较大直径血管（老年动物）中引起的血管收缩大于较小直径血管（幼年动物），提示存在年龄相关反应。选择性α2-激动剂可乐定（CLO）或赛拉嗪（XYL）仅产生轻微的血管收缩。酪胺（TYR）也仅产生轻微的血管收缩反应。诱导血管收缩的效能顺序为：EPI≥5-HT≥NE＞MT = PE＞＞KCl＞CLO = XYL = TYR。长时间冷保存对所有所用肾上腺素能激动剂和5-HT引起的血管收缩反应无显著影响。然而，冷保存显著抑制了氯化钾诱导的血管收缩。这些结果表明，猴骨骼肌动脉的节后α-肾上腺素能受体主要为α1型。由于冷保存显著抑制了氯化钾诱导的反应，但未影响肾上腺素能激动剂和5-HT引起的反应，因此认为冷保存可能严重损害了骨骼肌动脉血管平滑肌的钙内流机制。
