白细胞介素-20 通过 GSK3β/β-catenin 信号通路抑制 MC3T3-E1 细胞的成骨分化。
Interleukin-20 inhibits the osteogenic differentiation of MC3T3-E1 cells via the GSK3β/β-catenin signalling pathway.
机构信息
Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, China; Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, China.
Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, China; Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, China.
出版信息
Arch Oral Biol. 2021 May;125:105111. doi: 10.1016/j.archoralbio.2021.105111. Epub 2021 Mar 22.
OBJECTIVE
To investigate the effects of interleukin-20 (IL-20) on the osteogenic differentiation of MC3T3-E1 cells.
METHODS
The pre-osteoblast line MC3T3-E1 was treated with different concentrations of IL-20 (0, 2, 20 and 100 ng/mL), and the cell viability was detected by the CCK8 assay. To assess the influence of IL-20 on osteogenic differentiation, alkaline phosphatase (ALP) activity and Alizarin red staining were performed at predetermined times. The expression levels of Runt-related transcription factor 2 (RUNX2), Osterix (Osx), glycogen synthase kinase-3β (GSK-3β) and β-catenin were detected by qRT-PCR and Western blotting analyses. 5 nmol/L lithium chloride (LiCl) was used as GSK-3β inhibitor.
RESULTS
IL-20 promoted cell proliferation but decreased ALP activity and mineralization. Moreover, IL-20 downregulated the expression of RUNX2, Osx and β-catenin but upregulated the level of GSK-3β.
CONCLUSIONS
The results suggest that IL-20 could inhibit the osteogenic differentiation of MC3T3-E1 cells via the GSK3β/β-catenin signalling pathway.
目的
研究白细胞介素-20（IL-20）对 MC3T3-E1 细胞成骨分化的影响。
方法
用不同浓度的白细胞介素-20（0、2、20 和 100ng/ml）处理前成骨细胞系 MC3T3-E1，用 CCK8 法检测细胞活力。为了评估 IL-20 对成骨分化的影响，在预定时间进行碱性磷酸酶（ALP）活性和茜素红染色。通过 qRT-PCR 和 Western blot 分析检测 runt 相关转录因子 2（RUNX2）、osterix（Osx）、糖原合成酶激酶-3β（GSK-3β）和β-连环蛋白的表达水平。用 5nmol/L 氯化锂（LiCl）作为 GSK-3β 抑制剂。
结果
IL-20 促进细胞增殖，但降低 ALP 活性和矿化。此外，IL-20 下调 RUNX2、Osx 和 β-连环蛋白的表达，但上调 GSK-3β 的水平。
结论
结果表明，IL-20 可通过 GSK3β/β-连环蛋白信号通路抑制 MC3T3-E1 细胞的成骨分化。
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