在偶氮二甲酰胺存在的情况下，通过高效液相色谱法测定双缩脲。

The determination of biurea in the presence of azodicarbonamide by HPLC.

作者信息

Bechtold W E, Shopp G M, Cheng Y S

机构信息

Inhalation Toxicology Research Institute, Lovelace Biomedical and Environmental Research Institute, Albuquerque, New Mexico 87185.

出版信息

J Anal Toxicol. 1988 Mar-Apr;12(2):89-93. doi: 10.1093/jat/12.2.89.

DOI:10.1093/jat/12.2.89

PMID:3379928

Abstract

Azodicarbonamide (ADA), a solid blowing agent used in the manufacture of plastics, has been selected for inhalation toxicity testing by the National Toxicology Program. To test for decomposition of ADA during aerosolization, an HPLC method was developed to quantitate the relative amounts of one possible degradation product, biurea, in bulk samples and filter samples collected after aerosolization. The method uses a C18 column with 10-micron particles, UV monitoring at 190 nm for biurea and 425 nm for ADA, and a mobile phase of 100% water. Quantitation is with 14C-labeled biurea and ADA as external standards. The assay was validated by spiking bulk ADA with projected levels of 1, 2, and 3% biurea. Levels of biurea bound in both bulk and filter-collected aerosol samples of ADA were both 0.50%, with relative standard deviations of 13 and 26%, respectively.

摘要

偶氮二甲酰胺（ADA）是一种用于塑料制造的固体发泡剂，已被美国国家毒理学计划选定用于吸入毒性测试。为了测试雾化过程中ADA的分解情况，开发了一种高效液相色谱法（HPLC），用于定量雾化后收集的大量样品和过滤样品中一种可能的降解产物——双缩脲的相对含量。该方法使用具有10微米颗粒的C18柱，对双缩脲在190nm处进行紫外监测，对ADA在425nm处进行紫外监测，流动相为100%水。采用14C标记的双缩脲和ADA作为外标进行定量。通过在大量ADA中加入预计含量为1%、2%和3%的双缩脲来验证该分析方法。ADA的大量样品和过滤收集的气溶胶样品中双缩脲的结合水平均为0.50%，相对标准偏差分别为13%和26%。