建立了一株携带 POU5F1（OCT4）-2A-mCerulean3 敲入报告基因的普通狨猴胚胎干细胞系 CMES40-OC。
Generation of a common marmoset embryonic stem cell line CMES40-OC harboring a POU5F1 (OCT4)-2A-mCerulean3 knock-in reporter allele.
机构信息
Department of Physiology, School of Medicine, Keio University, Tokyo, Japan; Laboratory for Marmoset Neural Architecture, RIKEN Center for Brain Science, Saitama, Japan.
Department of Physiology, School of Medicine, Keio University, Tokyo, Japan.
出版信息
Stem Cell Res. 2021 May;53:102308. doi: 10.1016/j.scr.2021.102308. Epub 2021 Mar 24.
POU class 5 homeobox 1 (POU5F1, also known as OCT4) is critical for maintenance of pluripotency, germ cell fate, reprogramming into a pluripotent state, and early embryogenesis. We generated an embryonic stem cell (ESC) line of the common marmoset (Callithrix jacchus) harboring a heterozygous knock-in allele of OCT4-P2A-mCerulean-T2A-pac. The ESC line (CMES40-OC) will be valuable for investigation of primed/naïve pluripotency and germ cell fate. Homozygous OCT4 knock-in clones were generated but could not be sustained in an undifferentiated state in long-term culture. The OCT4 knock-in system facilitated simultaneous knock-in of a reporter construct at another locus, DDX4 (VASA).
POU 类 5 同源框 1（POU5F1，也称为 OCT4）对于维持多能性、生殖细胞命运、重编程为多能状态和早期胚胎发生至关重要。我们生成了一种携带 OCT4-P2A-mCerulean-T2A-pac 杂合敲入等位基因的普通狨猴（Callithrix jacchus）胚胎干细胞（ESC）系。该 ESC 系（CMES40-OC）将非常有助于研究初始/幼稚多能性和生殖细胞命运。生成了 OCT4 敲入纯合克隆，但在长期培养中不能在未分化状态下维持。OCT4 敲入系统促进了另一位点 DDX4（VASA）报告基因构建体的同时敲入。
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