University Lyon, University Lyon 1, INSERM, Stem Cell and Brain Research Institute U1208, 69500 Bron, France; Embryo Technology and Stem Cell Research Center, School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand.
Embryo Technology and Stem Cell Research Center, School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand.
Stem Cell Reports. 2023 Nov 14;18(11):2016-2037. doi: 10.1016/j.stemcr.2023.09.013. Epub 2023 Oct 19.
For nearly three decades, more than 80 embryonic stem cell lines and more than 100 induced pluripotent stem cell lines have been derived from New World monkeys, Old World monkeys, and great apes. In this comprehensive review, we examine these cell lines originating from marmoset, cynomolgus macaque, rhesus macaque, pig-tailed macaque, Japanese macaque, African green monkey, baboon, chimpanzee, bonobo, gorilla, and orangutan. We outline the methodologies implemented for their establishment, the culture protocols for their long-term maintenance, and their basic molecular characterization. Further, we spotlight any cell lines that express fluorescent reporters. Additionally, we compare these cell lines with human pluripotent stem cell lines, and we discuss cell lines reprogrammed into a pluripotent naive state, detailing the processes used to attain this. Last, we present the findings from the application of these cell lines in two emerging fields: intra- and interspecies embryonic chimeras and blastoids.
近三十年来，已经从新世界猴、旧世界猴和大猿中衍生出了 80 多个胚胎干细胞系和 100 多个诱导多能干细胞系。在这篇全面的综述中，我们研究了源自狨猴、食蟹猴、恒河猴、长尾猕猴、日本猕猴、绿长尾猴、狒狒、黑猩猩、倭黑猩猩、大猩猩和猩猩的这些细胞系。我们概述了建立这些细胞系所采用的方法、长期维持的培养方案以及它们的基本分子特征。此外，我们还重点介绍了表达荧光报告基因的细胞系。此外，我们还将这些细胞系与人类多能干细胞系进行了比较，并讨论了重编程为多能原始状态的细胞系，详细介绍了实现这一目标所使用的过程。最后，我们介绍了这些细胞系在两个新兴领域中的应用：种内和种间胚胎嵌合体和胚状体。
