Ngamdokmai Ngamrayu, Paracha Tamkeen Urooj, Waranuch Neti, Chootip Krongkarn, Wisuitiprot Wudtichai, Suphrom Nungruthai, Insumrong Kamonlak, Ingkaninan Kornkanok
Centre of Excellence in Cannabis Research, Department of Pharmaceutical Chemistry and Pharmacognosy, Faculty of Pharmaceutical Sciences and Center of Excellence for Innovation in Chemistry, Naresuan University, Phitsanulok 65000, Thailand.
Department of Pharmacology, Faculty of Pharmacy, Hamdard University, Main Campus, Karachi 74600, Pakistan.
Pharmaceuticals (Basel). 2021 Mar 11;14(3):253. doi: 10.3390/ph14030253.
Cellulite is associated with a complex array of adipocytes under the skin and vascular system. A herbal compress that was previously developed was proven to have an anti-cellulite effect in healthy volunteers within 2 weeks of treatment. However, its mechanism and ingredients responsible for reducing cellulite were not known. The purpose of this study was to investigate the activity of eight essential oils in, and two water extracts from, the ingredients of the herbal compress together with nine monoterpenoid constituents on the 3T3-L1 adipocytes. The vasodilatory effect on rat aortae was also studied. The adipocytes were induced by dexamethasone, 3-isobutyl-1-methylxanthine and insulin. At all concentrations tested, all essential oils, water extracts and their monoterpenoid constituents significantly inhibited lipid accumulation activity ( < 0.05) and decreased the amount of triglycerides when compared to untreated cells ( < 0.01). In addition, our results showed that the mixed oil distilled from the herbal compress mixed ingredients could relax the isolated rat aorta (EC = 14.74 ± 2.65 µg/mL). In conclusion, all essential oils, extracts and chemical constituents tested showed effects on adipogenesis inhibition and lipolysis induction on the cultured adipocytes with the mixed oil demonstrating vasorelaxation activity, all of which might be the mechanisms of the anti-cellulite effects of the herbal compress.
橘皮组织与皮肤下复杂的脂肪细胞阵列和血管系统有关。先前开发的一种草药热敷疗法在健康志愿者中经证实，在治疗2周内具有抗橘皮组织的效果。然而，其减少橘皮组织的机制和成分尚不清楚。本研究的目的是研究草药热敷疗法成分中的八种精油、两种水提取物以及九种单萜类成分对3T3-L1脂肪细胞的活性。还研究了它们对大鼠主动脉的血管舒张作用。脂肪细胞由地塞米松、3-异丁基-1-甲基黄嘌呤和胰岛素诱导产生。在所有测试浓度下，与未处理的细胞相比，所有精油、水提取物及其单萜类成分均显著抑制脂质积累活性（P<0.05）并降低甘油三酯含量（P<0.01）。此外，我们的结果表明，从草药热敷疗法混合成分中蒸馏出的混合油可使离体大鼠主动脉舒张（半数有效浓度=14.74±2.65µg/mL）。总之，所有测试的精油、提取物和化学成分均对培养的脂肪细胞表现出抑制脂肪生成和诱导脂肪分解的作用，混合油具有血管舒张活性，所有这些可能是草药热敷疗法抗橘皮组织作用的机制。
