Department of Oriental Medicine Resources and Institute of Korean Medicine Industry, Mokpo National University, Jeonnam 534-729, Republic of Korea.
J Integr Med. 2017 Jan;15(1):56-63. doi: 10.1016/S2095-4964(17)60321-2.
Traditional Korean Chungtaejeon (CTJ) tea is a type of fermented tea, which has received increasing attention in recent years because of its purported health benefits. The present study was designed to investigate the effect and mechanism of CTJ tea extract on body weight gain using C57BL/6J-Lep ob/ob mice and 3T3-L1 adipocytes, respectively.
The effects of CTJ on cell viability, lipid accumulation, and expression of protein and mRNA were measured in 3T3-L1 adipocytes by using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide, oil red O staining, Western blotting, and reverse transcriptase-polymerase chain reaction analyses. C57BL6J-Lep ob/ob mice were administered with CTJ (200 or 400 mg/kg body weight) for ten weeks. Then, body weight, food intake, total cholesterol, and triglyceride were measured in ob/ob mice.
CTJ tea extract treated at 250 μg/mL (CTJ250) significantly suppressed lipid accumulation in the differentiated 3T3-L1 adipocytes. Likewise, CTJ250 significantly decreased the protein expression of peroxisome proliferator-activated receptorγ (PPARγ), CCAAT/enhancer-binding protein α, and adipocyte lipid-binding protein, and regulated the mRNA expression of PPARγ, sterol regulatory element-binding protein-1c gene, fatty acid synthase, adipocyte lipid-binding protein, hormone-sensitive lipase, carnitine palmitoyl transferase 1, cluster of differentiation 36, and acetyl-CoA carboxylase in the differentiated 3T3-L1 adipocytes. Mice administered with CTJ showed dose-dependent decrease in body weight gain, starting from week 4 of the experiment. CTJ tea extract administered at 400 mg/kg body weight significantly decreased fat mass, food efficacy ratio, and levels of plasma triglyceride and total cholesterol.
CTJ attenuated weight gain in ob/ob mice and regulated the activity of the molecules involved in adipogenesis and lipolysis in 3T3-L1 adipocytes. CTJ is a potentially valuable herbal therapy for the prevention of obesity and/or obesity-related disorders.
传统的韩国忠太煎茶（CTJ）是一种发酵茶，近年来因其据称具有健康益处而受到越来越多的关注。本研究旨在分别使用 C57BL/6J-Lep ob/ob 小鼠和 3T3-L1 脂肪细胞来研究 CTJ 茶提取物对体重增加的影响及其机制。
通过 3-[4,5-二甲基噻唑-2-基]-2,5-二苯基四氮唑溴盐、油红 O 染色、Western 印迹和逆转录-聚合酶链反应分析，测量 CTJ 在 3T3-L1 脂肪细胞中的细胞活力、脂质积累以及蛋白和 mRNA 的表达。给 C57BL6J-Lep ob/ob 小鼠灌胃 CTJ（200 或 400mg/kg 体重）10 周。然后测量 ob/ob 小鼠的体重、食物摄入量、总胆固醇和甘油三酯。
在 250μg/mL（CTJ250）的 CTJ 茶提取物处理下，分化的 3T3-L1 脂肪细胞中的脂质积累明显受到抑制。同样，CTJ250 显著降低过氧化物酶体增殖物激活受体γ（PPARγ）、CCAAT/增强子结合蛋白α和脂肪细胞脂质结合蛋白的蛋白表达，并调节分化的 3T3-L1 脂肪细胞中 PPARγ、固醇调节元件结合蛋白-1c 基因、脂肪酸合酶、脂肪细胞脂质结合蛋白、激素敏感脂肪酶、肉毒碱棕榈酰转移酶 1、分化抗原 36 和乙酰辅酶 A 羧化酶的 mRNA 表达。给予 CTJ 的小鼠从实验第 4 周开始体重增加呈剂量依赖性下降。400mg/kg 体重的 CTJ 茶提取物显著降低脂肪质量、食物效率比以及血浆甘油三酯和总胆固醇水平。
CTJ 可减轻 ob/ob 小鼠的体重增加，并调节 3T3-L1 脂肪细胞中脂肪生成和脂肪分解相关分子的活性。CTJ 可能是预防肥胖和/或肥胖相关疾病的有价值的草药疗法。
