Hook G R, Hosseini J M, Elin R J
Surgical Neurology Branch, National Institutes of Neurological and Communicative Disorders and Stroke, Bethesda, MD.
Magnesium. 1988;7(1):1-5.
We present a mononuclear blood cell (MBC) isolation method for magnesium analysis by electron probe X-ray microanalysis. We compare the inorganic elemental composition of individual MBC isolated by either arabinogalactan (Stractan) or conventional Ficoll-Hypaque density gradients. We find that the MBC isolated with Stractan have the expected cellular element composition, but MBC isolated with Ficoll-Hypaque are contaminated with iodine and sodium. We discuss the source and significance of iodine and sodium contamination of Ficoll-Hypaque isolated MBC and recommend the Stractan separation method for electron probe X-ray microanalysis.
我们介绍了一种用于通过电子探针X射线微分析进行镁分析的单核血细胞（MBC）分离方法。我们比较了通过阿拉伯半乳聚糖（Stractan）或传统的Ficoll-Hypaque密度梯度分离的单个MBC的无机元素组成。我们发现，用Stractan分离的MBC具有预期的细胞元素组成，但用Ficoll-Hypaque分离的MBC被碘和钠污染。我们讨论了Ficoll-Hypaque分离的MBC中碘和钠污染的来源及意义，并推荐使用Stractan分离方法进行电子探针X射线微分析。
