Institute of Insect Science, Zhejiang University, Hangzhou 310058, China.
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of MOA of China and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, China.
Genes (Basel). 2021 Mar 7;12(3):379. doi: 10.3390/genes12030379.
(), a gene involved in the sex-determining cascade of , works in concert with the female-specific product of () at the end of the hierarchy to implement the sex-specific differentiation of sexually dimorphic characters in female individuals. In this study, the homolog was identified in the brown planthopper (BPH), , which contained two splice variants expressed in both female and male insects. We found that played a vital role in the early nymphal development of BPH, showing an accumulated effect. RNAi-mediated knockdown of at 4th instar led to the external genital defects in both sexes, consequently resulting in the loss of reproductive ability in female and male individuals. After dsRNA injection, the males were normal on testes, while the females had defective ovarian development. was also required for early embryogenesis. Notably, when the ds microinjection was performed in newly emerged females, the copulatory bursas were abnormally enlarged while the other tissues of the reproductive system developed normally. Our results demonstrated the pleiotropic roles of in embryogenesis and development of the reproductive system in a hemimetabolous insect species.
（），一个参与性别决定级联的基因，与性别特异性产物（）协同作用，在性别特异分化的性二态特征中发挥作用在女性个体中。在这项研究中，在褐飞虱（BPH）中鉴定出了 同源物，它包含两个剪接变体，在雌雄昆虫中均有表达。我们发现 在 BPH 的早期若虫发育中起着至关重要的作用，表现出累积效应。在第 4 龄期进行 RNAi 介导的敲低会导致两性的外生殖器缺陷，从而导致雌性和雄性个体丧失生殖能力。dsRNA 注射后，雄性睾丸正常，而雌性卵巢发育缺陷。 也需要早期胚胎发生。值得注意的是，当在新出现的雌性中进行 ds 微注射时，交配附器异常增大，而生殖系统的其他组织则正常发育。我们的结果表明了 在半变态昆虫物种的胚胎发生和生殖系统发育中的多效性作用。
