Nascimento Júllia A S, Santos Fernanda F, Valiatti Tiago B, Santos-Neto José F, M Santos Ana Carolina, Cayô Rodrigo, Gales Ana C, A T Gomes Tânia
Laboratório Experimental de Patogenicidade de Enterobactérias (LEPE), Disciplina de Microbiologia, Departamento de Microbiologia, Imunologia e Parasitologia (DMIP), Escola Paulista de Medicina (EPM), Universidade Federal de São Paulo (UNIFESP), São Paulo 04023-062, Brazil.
Laboratório Alerta, Disciplina de Infectologia, Departamento de Medicina, Escola Paulista de Medicina (EPM), Universidade Federal de São Paulo (UNIFESP), São Paulo 04039-032, Brazil.
Microorganisms. 2021 Mar 27;9(4):693. doi: 10.3390/microorganisms9040693.
(1) Background: Hybrid uropathogenic (UPEC) strains carry virulence markers of the diarrheagenic (DEC) pathotypes, which may increase their virulence potential. This study analyzed the frequency and virulence potential of hybrid strains among 452 UPEC strains. (2) Methods: Strains were tested for the DEC virulence diagnostic genes' presence by polymerase chain reaction (PCR). Those carrying at least one gene were classified as hybrid and further tested for 10 UPEC and extraintestinal pathogenic (ExPEC) virulence genes and phylogenetic classification. Also, their ability to produce hemolysis, adhere to HeLa and renal HEK 293T cells, form a biofilm, and antimicrobial susceptibility were evaluated. (3) Results: Nine (2%) hybrid strains were detected; seven of them carried and two, , and were classified as UPEC/EAEC (enteroaggregative ) and UPEC/aEPEC (atypical enteropathogenic ), respectively. They belonged to phylogroups A (five strains), B1 (three), and D (one), and adhered to both cell lineages tested. Only the UPEC/EAEC strains were hemolytic (five strains) and produced biofilm. One UPEC/aEPEC strain was resistant to third-generation cephalosporins and carried . (4) Conclusions: Our findings contribute to understanding the occurrence and pathogenicity of hybrid UPEC strains, which may cause more severe infections.
(1) 背景：杂交尿路致病性大肠杆菌（UPEC）菌株携带致泻性大肠杆菌（DEC）致病型的毒力标记，这可能会增加它们的毒力潜能。本研究分析了452株UPEC菌株中杂交菌株的频率和毒力潜能。(2) 方法：通过聚合酶链反应（PCR）检测菌株中DEC毒力诊断基因的存在情况。携带至少一个基因的菌株被归类为杂交菌株，并进一步检测10种UPEC和肠道外致病性大肠杆菌（ExPEC）毒力基因以及进行系统发育分类。此外，还评估了它们产生溶血、黏附于HeLa细胞和肾HEK 293T细胞、形成生物膜的能力以及抗菌药敏性。(3) 结果：检测到9株（2%）杂交菌株；其中7株携带 ，2株携带 ，分别被归类为UPEC/EAEC（肠集聚性大肠杆菌）和UPEC/aEPEC（非典型肠致病性大肠杆菌）。它们属于A群（5株）、B1群（3株）和D群（1株），并能黏附于所检测的两种细胞系。只有UPEC/EAEC菌株具有溶血能力（5株）并能形成生物膜。1株UPEC/aEPEC菌株对第三代头孢菌素耐药并携带 。(4) 结论：我们的研究结果有助于了解杂交UPEC菌株的发生情况和致病性，这些菌株可能会引起更严重的感染。
