Reichhardt Caleb C, Okamoto Lillian L, Motsinger Laura A, Griffin Brian P, Murdoch Gordon K, Thornton Kara J
Department of Animal, Dairy and Veterinary Science, Utah State University, 4815 Old Main Hill, Logan, UT 84322, USA.
Department of Animal, Veterinary and Food Sciences, University of Idaho, Logan, ID 83844, USA.
Animals (Basel). 2021 Mar 10;11(3):764. doi: 10.3390/ani11030764.
Emerging research suggests that hormones found in anabolic implants interact with polyamine biosynthesis. The objective of this study was to determine the effects of steroidal hormones, polyamines and polyamine precursors on bovine satellite cell (BSC) differentiation and polyamine biosynthesis temporally. Primary BSCs were induced to differentiate in 3% horse serum (CON) and treated with 10 nM trenbolone acetate (TBA), 10 nM estradiol (E2), 10 nM TBA and 10 nM E2, 10 mM methionine, 8 mM ornithine, 2 mM putrescine, 1.5 mM spermidine, or 0.5 mM spermine. Total mRNA was isolated 0, 2, 4, 8, 12, 24, and 48 h post-treatment. Abundance of mRNA for genes associated with induction of BSC differentiation: paired box transcription factor 7, myogenic factor 5, and myogenic differentiation factor 1 and genes in the polyamine biosynthesis pathway: ornithine decarboxylase and S-adenosylmethionine-were analyzed. Overall, steroidal hormones did not impact ( > 0.05) mRNA abundance of genes involved in BSC differentiation, but did alter ( = 0.04) abundance of genes involved in polyamine biosynthesis. Polyamine precursors influenced ( < 0.05) mRNA of genes involved in BSC differentiation. These results indicate that polyamine precursors and polyamines impact BSC differentiation and abundance of mRNA involved in polyamine biosynthesis, while steroidal hormones altered the mRNA involved in polyamine biosynthesis.
新出现的研究表明，合成代谢植入物中发现的激素与多胺生物合成相互作用。本研究的目的是确定甾体激素、多胺和多胺前体对牛卫星细胞（BSC）分化和多胺生物合成的时间影响。将原代BSC在3%马血清（CON）中诱导分化，并用10 nM醋酸群勃龙（TBA）、10 nM雌二醇（E2）、10 nM TBA和10 nM E2、10 mM蛋氨酸、8 mM鸟氨酸、2 mM腐胺、1.5 mM亚精胺或0.5 mM精胺处理。在处理后0、2、4、8、12、24和48小时分离总mRNA。分析与BSC分化诱导相关基因：配对盒转录因子7、生肌因子5和成肌分化因子1以及多胺生物合成途径中基因：鸟氨酸脱羧酶和S-腺苷甲硫氨酸的mRNA丰度。总体而言，甾体激素对参与BSC分化的基因的mRNA丰度没有影响（>0.05），但确实改变了（=0.04）参与多胺生物合成的基因的丰度。多胺前体影响（<0.05）参与BSC分化的基因的mRNA。这些结果表明，多胺前体和多胺影响BSC分化以及参与多胺生物合成的mRNA丰度，而甾体激素改变了参与多胺生物合成的mRNA。
