Department of Food Science and Biotechnology, Sejong University, 98 Gunja-dong, Seoul 143-747, Korea.
AMOREPACIFIC R&D Center, 1920, Yonggu-daero, Giheung-gu, Yongin-si 17074, Gyeonggi-do, Korea.
Molecules. 2021 Mar 10;26(6):1518. doi: 10.3390/molecules26061518.
The aim of this study was to profile the bioaccessibility and intestinal absorption of epicatechins and flavonols in different forms of green tea and its formulation: loose leaf tea, powdered tea, 35% catechins containing GTE, and GTE formulated with green tea-derived polysaccharide and flavonols (CATEPLUS™). The bioaccessibillity and intestinal absorption of epicatechins and flavonols was investigated by using an in vitro digestion model system with Caco-2 cells. The bioaccessibility of total epicatechins in loose leaf tea, powdered tea, GTE, and CATEPLUS™ was 1.27%, 2.30%, 22.05%, and 18.72%, respectively, showing that GTE and CATEPLUS™ had significantly higher bioaccessibility than powdered tea and loose leaf tea. None of the flavonols were detected in powdered tea and loose leaf tea, but the bioaccessibility of the total flavonols in GTE and CATEPLUS™ was 85.74% and 66.98%, respectively. The highest intestinal absorption of epicatechins was found in CATEPLUS™ (171.39 ± 5.39 ng/mg protein) followed by GTE (57.38 ± 9.31), powdered tea (3.60 ± 0.67), and loose leaf tea (2.94 ± 1.03). The results from the study suggest that formulating green tea extracts rich in catechins with second components obtained from green tea processing could enhance the bioavailability of epicatechins.
本研究旨在分析不同形态的绿茶及其配方（散叶茶、茶粉、含 35%儿茶素的 GTE 和用绿茶来源多糖和黄酮醇制成的 GTE）中表儿茶素和黄酮醇的生物利用度和肠道吸收情况。采用 Caco-2 细胞体外消化模型系统研究了表儿茶素和黄酮醇的生物利用度和肠道吸收情况。散叶茶、茶粉、GTE 和 CATEPLUS™中总表儿茶素的生物利用度分别为 1.27%、2.30%、22.05%和 18.72%，表明 GTE 和 CATEPLUS™的生物利用度明显高于茶粉和散叶茶。茶粉和散叶茶中均未检测到黄酮醇，但 GTE 和 CATEPLUS™中总黄酮醇的生物利用度分别为 85.74%和 66.98%。表儿茶素的肠道吸收率最高的是 CATEPLUS™（171.39±5.39ng/mg 蛋白），其次是 GTE（57.38±9.31）、茶粉（3.60±0.67）和散叶茶（2.94±1.03）。研究结果表明，用绿茶加工过程中获得的第二成分来配方富含儿茶素的绿茶提取物，可以提高表儿茶素的生物利用度。
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