Camargo L E A, Pedroso L S, Vendrame S C, Mainardes R M, Khalil N M
Pharmacy Department, Faculdade Guairacá, Guarapuava, PR, Brazil.
Laboratory of Pharmaceutical Nanotechnology, Universidade Estadual do Centro-Oeste, Guarapuava, PR, Brazil.
Braz J Biol. 2016 Jun;76(2):428-34. doi: 10.1590/1519-6984.18814. Epub 2016 Mar 15.
The antioxidant and anticandidal activities of leaves obtained from Camellia sinensis by non-fermentation (green and white teas), semi-fermentation (red tea) and fermentation method (black tea) were investigated. It was evaluated the total phenolic content by Folin-Ciocalteau assay; antioxidant capacities were evaluated in vitro using DPPH and ABTS radicals, hypochlorous acid and superoxide anion scavenger assays, induced hemolysis, lipid peroxidation by conjugated diene formation and myeloperoxidase activity. Anticandidal activity was performed on three strains of Candida spp. The results showed that non-fermented teas have a higher concentration of phenolic compounds, and then presented the best inhibitory activity of AAPH-induced hemolysis, the best inhibition of conjugated diene formation and more pronounced antioxidant activity in all tests. The highest anticandidal activity was obtained from fermented tea, followed by non-fermented tea. These results indicate that the antioxidant activity demonstrated has no direct relation with the anticandidal activity.
研究了通过非发酵(绿茶和白茶)、半发酵(红茶)和发酵方法(黑茶)从茶树获得的叶片的抗氧化和抗念珠菌活性。通过福林-西奥尔特法测定总酚含量;使用DPPH和ABTS自由基、次氯酸和超氧阴离子清除剂测定、诱导溶血、共轭二烯形成引起的脂质过氧化和髓过氧化物酶活性在体外评估抗氧化能力。对三种念珠菌属菌株进行抗念珠菌活性测试。结果表明,非发酵茶的酚类化合物浓度较高,在所有测试中表现出对AAPH诱导溶血的最佳抑制活性、对共轭二烯形成的最佳抑制以及更显著的抗氧化活性。发酵茶获得最高的抗念珠菌活性,其次是非发酵茶。这些结果表明,所显示的抗氧化活性与抗念珠菌活性没有直接关系。