Wong N L, Sutton R A, Dirks J H
Department of Medicine, Health Sciences Centre Hospital, University of British Columbia, Vancouver, Canada.
Nephron. 1988;49(1):62-6. doi: 10.1159/000184988.
Clearance and in vitro microperfusion studies were performed in rabbits to determine the effect of cisplatin on proximal straight tubule transport of calcium and magnesium. Rabbits were injected with cisplatin (2.5 mg/kg i.p. once weekly) for 3 weeks, whereas control rabbits received normal saline solution which served as a diluent for cisplatin. In 5 rabbits, 24-hour clearance studies were performed with the aid of a metabolic cage. Following cisplatin treatment, fractional excretion of magnesium rose significantly (73.3 +/- 11.5 vs. 111.4 +/- 17.5%). Glomerular filtration rate fell with cisplatin treatment (4.05 +/- 0.76 vs. 2.81 +/- 28 ml/min). There was no difference in fractional excretion of calcium (26.3 +/- 9.5 vs. 22.7 +/- 3.2%). The cortical and juxtamedullary proximal straight tubules were perfused in vitro. Net volume absorption was the same in the control and cisplatin-treated rabbits. However, there was a significant reduction in JCa (cortical 0.57 +/- 0.10 vs. -0.10 +/- 0.12 pmol/min/mm; juxtamedullary 0.96 +/- 0.17 vs. 0.31 +/- 0.37 pmol/min/mm) and JMg (cortical 0.43 +/- 0.08 vs. -0.15 +/- 0.07 pmol/min/mm; juxtamedullary 0.40 +/- 0.27 vs. -0.30 +/- 0.28 pmol/min/mm). In contrast to chronic administration, acute addition of cisplatin into the bath had no effect on JCa and JMg in the cortical and juxtamedullary proximal straight tubules. These data indicate that chronic but not acute cisplatin treatment depresses the transport of calcium and magnesium in the cortical and juxtamedullary nephrons of the proximal straight tubule of the rabbit.
在兔子身上进行了清除率和体外微灌注研究，以确定顺铂对近端直小管钙和镁转运的影响。兔子每周一次腹腔注射顺铂（2.5mg/kg），共注射3周，而对照兔子则接受用作顺铂稀释剂的生理盐水溶液。对5只兔子借助代谢笼进行了24小时清除率研究。顺铂治疗后，镁的分数排泄显著升高（73.3±11.5对111.4±17.5%）。顺铂治疗后肾小球滤过率下降（4.05±0.76对2.81±28ml/min）。钙的分数排泄没有差异（26.3±9.5对22.7±3.2%）。对皮质和近髓质近端直小管进行了体外灌注。对照兔子和经顺铂处理的兔子的净体积吸收相同。然而，JCa（皮质：0.57±0.10对-0.10±0.12pmol/min/mm；近髓质：0.96±0.17对0.31±0.37pmol/min/mm）和JMg（皮质：0.43±0.08对-0.15±0.07pmol/min/mm；近髓质：0.40±0.27对-0.30±0.28pmol/min/mm）显著降低。与慢性给药相反，向浴槽中急性添加顺铂对皮质和近髓质近端直小管的JCa和JMg没有影响。这些数据表明，慢性而非急性顺铂治疗会抑制兔子近端直小管皮质和近髓质肾单位中钙和镁的转运。
