National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Thailand Science Park, Pathumthani 12120, Thailand.
Section of Translational Medicine, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok 10400, Thailand.
Biosensors (Basel). 2021 Mar 17;11(3):85. doi: 10.3390/bios11030085.
An immobilization-free electrochemical sensor coupled with a graphene oxide (GO)-based aptasensor was developed for glycated human serum albumin (GHSA) detection. The concentration of GHSA was monitored by measuring the electrochemical response of free GO and aptamer-bound GO in the presence of glycated albumin; their currents served as the analytical signals. The electrochemical aptasensor exhibited good performance with a base-10 logarithmic scale. The calibration curve was achieved in the range of 0.01-50 µg/mL. The limit of detection (LOD) was 8.70 ng/mL. The developed method was considered a one-drop measurement process because a fabrication step and the probe-immobilization process were not required. This simple sensor offers a cost-effective, rapid, and sensitive detection method, and could be an alternative approach for determination of GHSA levels.
一种免固定的电化学传感器与基于氧化石墨烯（GO）的适体传感器偶联，用于糖化人血清白蛋白（GHSA）的检测。通过测量存在糖化白蛋白时游离 GO 和适体结合的 GO 的电化学响应，监测 GHSA 的浓度；它们的电流作为分析信号。电化学适体传感器在以 10 为底的对数标度下表现出良好的性能。校准曲线在 0.01-50 µg/mL 的范围内。检测限（LOD）为 8.70 ng/mL。所开发的方法被认为是一滴测量过程，因为不需要制造步骤和探针固定过程。这种简单的传感器提供了一种经济高效、快速和灵敏的检测方法，可作为测定 GHSA 水平的替代方法。
