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地方品种的简化基因组测序及其在评估分类学关系中的应用

Genotyping-by-Sequencing in Landraces and Its Utility for Assessing Taxonomic Relationships.

作者信息

Zuluaga Diana Lucia, Lioi Lucia, Delvento Chiara, Pavan Stefano, Sonnante Gabriella

机构信息

Institute of Biosciences and Bioresources (IBBR), National Research Council (CNR), via Amendola 165/A, 70126 Bari, Italy.

Department of Soil, Plant and Food Science, University of Bari "Aldo Moro", via Amendola 165/A, 70126 Bari, Italy.

出版信息

Plants (Basel). 2021 Mar 9;10(3):509. doi: 10.3390/plants10030509.

DOI:10.3390/plants10030509
PMID:33803432
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8001400/
Abstract

Genotyping by sequencing (GBS) was used to analyze relationships among cowpea and asparagus bean landraces from southern Italy and to assess the utility of this technology to study taxonomy in a wider panel, including cultigroups, subspecies, and other species. The analysis of SNPs derived from GBS highlighted that, among the cowpea landraces, the African samples were separated from the other material, while, for the Italian landraces, a certain clustering depending on seed color/pattern was observed in the dendrogram. When examining the species complex, a clear separation between the two groups of wild subspecies, i.e., the allogamous wild perennials and the perennial out/inbreds, could be observed, the former representing the more ancestral wild progenitors of . The species appeared more closely related to than to the other species analyzed.

摘要

通过测序进行基因分型(GBS)被用于分析来自意大利南部的豇豆和长豇豆地方品种之间的关系,并评估该技术在更广泛的样本组(包括栽培群体、亚种和其他物种)中研究分类学的效用。对GBS衍生的单核苷酸多态性(SNP)的分析突出表明,在豇豆地方品种中,非洲样本与其他材料分开,而对于意大利地方品种,在聚类图中观察到根据种子颜色/图案有一定的聚类。在检查物种复合体时,可以观察到两组野生亚种,即异花授粉的野生多年生植物和多年生远交/近交植物之间有明显的分离,前者代表了……的更原始的野生祖先。该物种与……的关系似乎比与分析的其他……物种更密切。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1944/8001400/1d802732a985/plants-10-00509-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1944/8001400/932d0c7e914e/plants-10-00509-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1944/8001400/89805f7fa18e/plants-10-00509-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1944/8001400/a8fa8dd612ed/plants-10-00509-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1944/8001400/1d802732a985/plants-10-00509-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1944/8001400/932d0c7e914e/plants-10-00509-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1944/8001400/89805f7fa18e/plants-10-00509-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1944/8001400/a8fa8dd612ed/plants-10-00509-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1944/8001400/1d802732a985/plants-10-00509-g004.jpg

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本文引用的文献

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2
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Sci Rep. 2019 Aug 12;9(1):11719. doi: 10.1038/s41598-019-48239-5.
3
The genome assembly of asparagus bean, Vigna unguiculata ssp. sesquipedialis.
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Biotechnological Advances to Improve Abiotic Stress Tolerance in Crops.生物技术在提高作物非生物胁迫耐受性方面的进展。
Int J Mol Sci. 2022 Oct 10;23(19):12053. doi: 10.3390/ijms231912053.
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Plant Methods. 2022 Aug 29;18(1):106. doi: 10.1186/s13007-022-00938-3.
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