Bulavaitė Aistė, Maier Thomas, Pleckaityte Milda
Institute of Biotechnology, Life Sciences Center, Vilnius University, Sauletekio al. 7, 10257 Vilnius, Lithuania.
R&D Bioanalytics, MALDI Biotyper Business Area Microbiology & Diagnostics, Bruker Daltonik GmbH, Fahrenheitstr. 4, 28359 Bremen, Germany.
Pathogens. 2021 Mar 1;10(3):277. doi: 10.3390/pathogens10030277.
The description of was recently updated and three new species, including nine genome species within , were defined using whole genome sequences and matrix assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry. A fast and simple method based on readily available techniques would be of immense use to identify species in research and clinical practice. Here we show that 34 previously characterized isolates were assigned to the species using partial chaperonin sequences. The MALDI Biotyper from Bruker Daltonik GmbH demonstrated the capability to differentiate the phylogenetically diverse groups composed of / and /. Among the phenotypic properties that characterize species are sialidase and β-galactosidase activities. Our data confirmed that the NanH3 enzyme is responsible for sialidase activity in spp. isolates. Almost all isolates displayed a sialidase positive phenotype, whereas the majority of strains were sialidase negative. and displayed a sialidase negative phenotype. β-galactosidase is produced exclusively in . strains. Earlier determined phenotypic characteristics associated with virulence of isolates now assigned to the defined species may provide insights on how diverse species contribute to shaping the vaginal microbiome.
最近对 的描述进行了更新，利用全基因组序列和基质辅助激光解吸电离飞行时间（MALDI-TOF）质谱法定义了三个新物种，包括 内的九个基因组物种。基于现有技术的快速简便方法对于在研究和临床实践中鉴定 物种将非常有用。在这里，我们表明，使用部分伴侣蛋白 序列将34个先前鉴定的 分离株归为该物种。布鲁克道尔顿公司的MALDI Biotyper证明了区分由 / 和 / 组成的系统发育不同群体的能力。表征 物种的表型特性包括唾液酸酶和β-半乳糖苷酶活性。我们的数据证实，NanH3酶负责 spp. 分离株中的唾液酸酶活性。几乎所有 分离株都表现出唾液酸酶阳性表型，而大多数 菌株为唾液酸酶阴性。 和 表现出唾液酸酶阴性表型。β-半乳糖苷酶仅在 菌株中产生。现在分配到已定义物种的 分离株的早期确定的与毒力相关的表型特征可能为不同物种如何影响阴道微生物群的形成提供见解。
