Kushawaha Akhilesh Kumar, Khan Ambreen, Sopory Sudhir Kumar, Sanan-Mishra Neeti
Plant RNAi Biology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110067, India.
Life (Basel). 2021 Mar 29;11(4):291. doi: 10.3390/life11040291.
Rice plants often encounter high temperature stress, but the associated coping strategies are poorly understood. It is known that a prior shorter exposure to high temperature, called thermo-priming, generally results in better adaptation of the plants to subsequent exposure to high temperature stress. High throughput sequencing of transcript and small RNA libraries of rice seedlings primed with short exposure to high temperature followed by high temperature stress and from plants exposed to high temperature without priming was performed. This identified a number of transcripts and microRNAs (miRs) that are induced or down regulated. Among them osa-miR531b, osa-miR5149, osa-miR168a-5p, osa-miR1846d-5p, osa-miR5077, osa-miR156b-3p, osa-miR167e-3p and their respective targets, coding for heat shock activators and repressors, showed differential expression between primed and non-primed plants. These findings were further validated by qRT-PCR. The results indicate that the miR-regulated heat shock proteins (HSPs)/heat shock transcription factors (HSFs) may serve as important regulatory nodes which are induced during thermo-priming for plant survival and development under high temperatures.
水稻经常遭遇高温胁迫，但其相关应对策略却鲜为人知。已知先前较短时间暴露于高温（即热引发）通常会使植株对随后的高温胁迫产生更好的适应性。对经短期高温引发后再遭受高温胁迫的水稻幼苗以及未经引发直接遭受高温的水稻幼苗的转录本和小RNA文库进行了高通量测序。这鉴定出了许多被诱导或下调的转录本和微小RNA（miR）。其中，osa-miR531b、osa-miR5149、osa-miR168a-5p、osa-miR1846d-5p、osa-miR5077、osa-miR156b-3p、osa-miR167e-3p及其各自编码热激激活因子和抑制因子的靶标，在引发植株和未引发植株之间表现出差异表达。这些发现通过qRT-PCR得到了进一步验证。结果表明，miR调控的热激蛋白（HSP）/热激转录因子（HSF）可能作为重要的调控节点，在热引发过程中被诱导，以利于植株在高温下的存活和发育。
