在多胺存在的情况下，雌激素受体对聚（dA-dC）·聚（dG-dT）的相对亲和力增加了22倍。
A twenty-two-fold increase in the relative affinity of estrogen receptor to poly (dA-dC).poly (dG-dT) in the presence of polyamines.
作者信息
Thomas T, Kiang D T
机构信息
Department of Medicine, University of Minnesota Hospital and Clinic, University of Minnesota, Minneapolis 55455.
出版信息
Nucleic Acids Res. 1988 May 25;16(10):4705-20. doi: 10.1093/nar/16.10.4705.
We studied the relative efficacy of polyamines to facilitate the binding of estrogen receptor to poly(dA-dC).poly(dG-dT). In the absence of polyamines, 1,400 micrograms/ml of this polynucleotide eluted 50% of bound estrogen receptor from DNA-cellulose. In contrast, 50% estrogen receptor was eluted by 65 micrograms/ml of poly(dA-dC).poly(dG-dT) complexed with 150 microM spermidine. Putrescine and spermine also enhanced the ability of poly(dA-dC).poly(dG-dT) to elute estrogen receptor, but the magnitude of the effect was not as high as that of spermidine. Control experiments with calf thymus DNA and poly(dA-dT).poly(dA-dT) showed 6- and 3-fold increase, respectively in their affinity for estrogen receptor in the presence of spermidine. The dramatic increase in the affinity of poly(dA-dC).poly(dG-dT) for estrogen receptor in the presence of polyamines might be a result of the conversion of the polynucleotide to the left-handed Z-DNA form. These results show that polyamines are capable of participating in estrogenic regulation of gene expression by altering the affinity of the receptor for specific DNA sequences.
我们研究了多胺促进雌激素受体与聚（dA-dC）·聚（dG-dT）结合的相对功效。在没有多胺的情况下，1400微克/毫升这种多核苷酸从DNA纤维素上洗脱了50%结合的雌激素受体。相比之下，与150微摩尔亚精胺复合的65微克/毫升聚（dA-dC）·聚（dG-dT）洗脱了50%的雌激素受体。腐胺和精胺也增强了聚（dA-dC）·聚（dG-dT）洗脱雌激素受体的能力，但效果程度不如亚精胺高。用小牛胸腺DNA和聚（dA-dT）·聚（dA-dT）进行的对照实验表明，在有亚精胺存在时，它们对雌激素受体的亲和力分别增加了6倍和3倍。在有多胺存在的情况下，聚（dA-dC）·聚（dG-dT）对雌激素受体亲和力的显著增加可能是多核苷酸转化为左手性Z-DNA形式的结果。这些结果表明，多胺能够通过改变受体对特定DNA序列的亲和力来参与基因表达的雌激素调节。
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