一种由多胺诱导产生的聚（氨基-2dA-dT）的A形式。聚（氨基-2dA-dT）。
An A-form of poly(amino2dA-dT).poly(amino2dA-dT) induced by polyamines.
作者信息
Garriga P, Garcia-Quintana D, Sági J, Manyosa J
机构信息
Departament de Bioquímica i de Biologia Molecular, Facultat de Medicina, Universitat Autònoma de Barcelona, Bellaterra, Spain.
出版信息
Biochemistry. 1993 Feb 2;32(4):1067-71. doi: 10.1021/bi00055a011.
The effect of the naturally occurring polyamines spermidine and spermine on poly(amino2-dA-dT).poly(amino2dA-dT) conformation has been studied by UV, CD, and IR spectroscopies. It is shown that a conformational transition is induced in poly(amino2dA-dT).poly(amino2dA-dT) by micromolar concentrations of the polyamines (30 microM) in low-salt aqueous solution. The analysis of our results, in view of previously published studies on conformational properties of the amino polynucleotide, indicates the resulting conformer to be an A-form. Interestingly, the polyamine concentration at the midpoint of the transition is the same in both cases. This provides further evidence that the coordination of positively charged counterions to DNA is determined largely from the DNA structure, probably with an important role for the sequence, and less from the nature of the ions.
通过紫外光谱、圆二色光谱和红外光谱研究了天然存在的多胺亚精胺和精胺对聚（氨基-2-dA-dT）·聚（氨基-2-dA-dT）构象的影响。结果表明，在低盐水溶液中，微摩尔浓度（30微摩尔）的多胺可诱导聚（氨基-2-dA-dT）·聚（氨基-2-dA-dT）发生构象转变。鉴于之前发表的关于氨基多核苷酸构象性质的研究，对我们的结果进行分析表明，所得构象为A 型。有趣的是，两种情况下转变中点的多胺浓度相同。这进一步证明，带正电荷的抗衡离子与DNA的配位作用在很大程度上取决于DNA结构，可能序列起重要作用，而离子性质的影响较小。
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