Forin Niccolò, Vizzini Alfredo, Fainelli Federico, Ercole Enrico, Baldan Barbara
Botanical Garden, University of Padova, Via Orto Botanico, 15, 35123 Padova, Italy.
Institute for Sustainable Plant Protection (IPSP-SS Torino), C.N.R., Viale P.A. Mattioli, 25, 10125 Torino, Italy.
Microorganisms. 2021 Mar 23;9(3):666. doi: 10.3390/microorganisms9030666.
In a recent monograph on the genus , type specimens worldwide were revised and re-classified using a morphological approach. Among them, some came from Pier Andrea Saccardo's fungarium stored in the Herbarium of the Padova Botanical Garden. In this work, we taxonomically re-examine via a morphological and molecular approach nine different Saccardo types. ITS1 and/or ITS2 sequences were successfully obtained applying Illumina MiSeq technology and phylogenetic analyses were carried out in order to elucidate their current taxonomic position. Only the ITS1 sequence was recovered for , while for , only the ITS2 sequence was recovered. We proposed here new combinations for , and , while for , , , and , we did not suggest taxonomic changes compared to the current ones. The name Sacc. is invalid, as it is a later homonym of P. Karst. & Starbäck. Therefore, we introduced as a nomen novum for Sacc. This is the first time that these types have been subjected to a molecular study. Our results demonstrate that old types are an important source of DNA sequence data for taxonomic re-examinations.
在最近一篇关于该属的专题论文中,使用形态学方法对全球范围内的模式标本进行了修订和重新分类。其中一些标本来自保存在帕多瓦植物园标本馆的皮尔·安德里亚·萨卡尔多真菌标本室。在这项工作中,我们通过形态学和分子学方法对九种不同的萨卡尔多类型进行了分类学重新研究。应用Illumina MiSeq技术成功获得了ITS1和/或ITS2序列,并进行了系统发育分析,以阐明它们当前的分类地位。对于[具体物种1],仅获得了ITS1序列,而对于[具体物种2],仅获得了ITS2序列。我们在此提出了[具体物种1]、[具体物种2]和[具体物种3]的新组合,而对于[具体物种4]、[具体物种5]、[具体物种6]、[具体物种7]和[具体物种8],与当前分类相比,我们未建议进行分类学更改。名称[具体名称1] Sacc.无效,因为它是[具体名称2] P. Karst. & Starbäck的后选同名异物。因此,我们引入[具体名称3]作为[具体名称1] Sacc.的新名称。这是首次对这些类型进行分子研究。我们的结果表明,旧类型是用于分类学重新研究的DNA序列数据的重要来源。