Nowak Bartosz, Kawka Mateusz, Wierzchowski Kamil, Sykłowska-Baranek Katarzyna, Pilarek Maciej
Faculty of Chemical and Process Engineering, Warsaw University of Technology, Waryńskiego 1, 00-645 Warsaw, Poland.
Department of Pharmaceutical Biology and Medicinal Plant Biotechnology, Faculty of Pharmacy, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland.
J Funct Biomater. 2021 Mar 5;12(1):19. doi: 10.3390/jfb12010019.
Unique biosynthetic abilities revealed by plants determine in vitro cultures of hairy roots as a suitable source of pharmaceutically relevant bioactive compounds. The basic aim of the study was to examine the applicability of aerogel composed of methyltrimethoxysilane (MTMS) for immobilization of hairy roots by identifying quantitative effects of biomass proliferation and naphthoquinones extracellular secretion in the aerogel-supported culture system. hairy roots were simultaneously cultured for 28-days, as (i) nonimmobilized biomass (reference system), (ii) biomass immobilized on macroporous polyurethane foam (PUF), (iii) biomass with disintegrated MTMS aerogel, (iv) biomass immobilized on polypropylene (PP) fibers (as control), and (v) biomass immobilized on monolithic PP-reinforced MTMS aerogel. MTMS aerogel exhibited high level of biocompatibility toward hairy roots which grew into the structure of monolithic aerogel-based constructs. Monolithic MTMS-based constructs significantly promoted the proliferation of hairy roots, resulting in 55% higher fresh mass than the reference system. The highest level of naphthoquinones productivity, i.e., 653 µg g, was noted for PUF-supported culture system.
植物所展现出的独特生物合成能力决定了毛状根的体外培养是药学相关生物活性化合物的合适来源。该研究的基本目的是通过确定气凝胶支持的培养系统中生物量增殖和萘醌细胞外分泌的定量影响，来检验由甲基三甲氧基硅烷（MTMS）组成的气凝胶用于固定毛状根的适用性。将毛状根同时培养28天，培养方式如下：（i）非固定化生物量（参考系统），（ii）固定在大孔聚氨酯泡沫（PUF）上的生物量，（iii）与分解的MTMS气凝胶混合的生物量，（iv）固定在聚丙烯（PP）纤维上的生物量（作为对照），以及（v）固定在整体式PP增强MTMS气凝胶上的生物量。MTMS气凝胶对生长到整体式气凝胶基构建体结构中的毛状根表现出高度的生物相容性。基于整体式MTMS的构建体显著促进了毛状根的增殖，其鲜质量比参考系统高55%。PUF支持的培养系统萘醌生产率最高，即653μg/g。
