Plaksin D, Gelber C, Feldman M, Eisenbach L
Department of Cell Biology, Weizmann Institute of Science, Rehovot, Israel.
Proc Natl Acad Sci U S A. 1988 Jun;85(12):4463-7. doi: 10.1073/pnas.85.12.4463.
High metastatic clones of the murine 3LL carcinoma express greatly reduced levels of H-2Kb major histocompatibility complex class I antigens, while low metastatic clones of the same tumor express high levels of H-2Kb. Induced expression of this antigen after transfection with the H-2Kb gene resulted in conversion of a metastatic to a non- or low-metastatic phenotype. Unlike the parental cells, transfected cells are potent inducers of H-2Kb-restricted syngeneic cytotoxic lymphocytes that kill the Kb-positive clones and cross-react with parental nontransfected cells. Preimmunization of mice with Kb-positive transfectants conferred protection against metastatic spread of malignant cells. Moreover, immunotherapy of metastasis was achieved by immunization with the H-2Kb-transfected cells of animals already carrying a growing local tumor of the parental cells.
小鼠3LL癌的高转移克隆表达的H-2Kb主要组织相容性复合体I类抗原水平大幅降低，而同一肿瘤的低转移克隆则表达高水平的H-2Kb。用H-2Kb基因转染后该抗原的诱导表达导致转移性表型转变为非转移或低转移表型。与亲代细胞不同，转染细胞是H-2Kb限制性同基因细胞毒性淋巴细胞的有效诱导剂，可杀死Kb阳性克隆并与亲代未转染细胞发生交叉反应。用Kb阳性转染子对小鼠进行预免疫可预防恶性细胞的转移扩散。此外，通过用已携带亲代细胞局部生长肿瘤的动物的H-2Kb转染细胞进行免疫来实现转移的免疫治疗。
