Ghiman Raluca, Nistor Madalina, Focșan Monica, Pintea Adela, Aștilean Simion, Rugina Dumitrita
Nanobiophotonics and Laser Microspectroscopy Center, Interdisciplinary Research Institute in Bio-Nano-Sciences, Babes-Bolyai University, 42 Treboniu Laurean, 400271 Cluj-Napoca, Romania.
Biochemistry Department, Faculty of Veterinary Medicine, University of Agricultural Science and Veterinary Medicine, 3-5 Calea Manastur, 400372 Cluj-Napoca, Romania.
Nanomaterials (Basel). 2021 Mar 19;11(3):782. doi: 10.3390/nano11030782.
Over the past decades, there has been a growing interest in using natural molecules with therapeutic potential for biomedical applications. In this context, our aim is focused on anthocyanins (AN) as molecules with anticancer properties that could be used in melanoma local therapies. Due to their susceptibility to environmental changes, current study is based on the design and development of a fluorescent system for carrying and trafficking AN inside melanoma cells. The architectural structure of the proposed system CaCO(PAH)@RBITC@AN reflects a spherical shape, 1080 nm diameter and a solid groundwork CaCO(PAH), on which rhodamine B isothiocyanate (RBITC) fluorophore was firstly added; then, poly(acrylic acid) (PAA) polyelectrolytes and poly(allylamine hydrochloride) (PAH) were successfully deposited. Purified AN from chokeberries were entrapped between PAA layers (rate of 94.6%). In vitro tests confirmed that CaCO(PAH)@RBITC@AN does not affect the proliferation of melanoma B16-F10 cells and proved that their internalization and trafficking can be followed after 24 h of treatment. Data presented here could contribute not only to the existing knowledge about the encapsulation technology of AN but also might bring relevant information for a novel formula to deliver therapeutic molecules or other bio-imaging agents directly into melanoma cells, a strategy that could positively improve tumor therapies.
在过去几十年中，人们对使用具有治疗潜力的天然分子用于生物医学应用的兴趣与日俱增。在此背景下，我们的目标聚焦于花青素（AN），它作为具有抗癌特性的分子，可用于黑色素瘤的局部治疗。由于它们对环境变化敏感，当前的研究基于一种荧光系统的设计与开发，该系统用于在黑色素瘤细胞内携带和运输AN。所提出的系统CaCO(PAH)@RBITC@AN的结构呈现球形，直径为1080 nm，其基础是CaCO(PAH)固体，首先在其上添加了异硫氰酸罗丹明B（RBITC）荧光团；然后，成功沉积了聚（丙烯酸）（PAA）聚电解质和聚（烯丙胺盐酸盐）（PAH）。从黑加仑中纯化得到的AN被困在PAA层之间（捕获率为94.6%）。体外试验证实CaCO(PAH)@RBITC@AN不会影响黑色素瘤B16 - F10细胞的增殖，并证明在处理24小时后可以追踪它们的内化和运输情况。此处呈现的数据不仅有助于丰富关于AN包封技术的现有知识，还可能为将治疗分子或其他生物成像剂直接递送至黑色素瘤细胞的新型配方带来相关信息，这一策略有望积极改善肿瘤治疗效果。
