Chair of Biopharmaceutics and Pharmacokinetics, Faculty of Pharmacy, University of Ljubljana, Aškerčeva 7, 1000 Ljubljana, Slovenia.
Molecules. 2021 Mar 22;26(6):1787. doi: 10.3390/molecules26061787.
Monoclonal antibodies (mAbs) have been extensively developed over the past few years, for the treatment of various inflammatory diseases. They are large molecules characterized by complex pharmacokinetic and pharmacodynamic properties. Therapeutic drug monitoring (TDM) is routinely implemented in the therapy with mAbs, to monitor patients' treatment response and to further guide dose adjustments. Serum has been the matrix of choice in the TDM of mAbs and its sampling requires the visit of the patients to laboratories that are not always easily accessible. Therefore, dried blood spots (DBS) and various microsampling techniques have been suggested as an alternative. DBS is a sampling technique in which capillary blood is deposited on a special filter paper. It is a relatively simple procedure, and the patients can perform the home-sampling. The convenience it offers has enabled its use in the quantification of small-molecule drugs, whilst in the recent years, studies aimed to develop microsampling methods that will facilitate the TDM of mAbs. Nevertheless, hematocrit still remains an obstacle that hinders a more widespread implementation of DBS in clinical practice. The introduction of novel analytical techniques and contemporary microsampling devices can be considered the steppingstone to the attempts made addressing this issue.
单克隆抗体（mAbs）在过去几年中得到了广泛的发展，用于治疗各种炎症性疾病。它们是具有复杂药代动力学和药效动力学特性的大分子。治疗药物监测（TDM）在 mAbs 的治疗中通常实施，以监测患者的治疗反应，并进一步指导剂量调整。血清一直是 mAbs TDM 的首选基质，其采样需要患者前往实验室，而这些实验室并不总是容易到达。因此，已经提出了干血斑（DBS）和各种微采样技术作为替代方法。DBS 是一种采样技术，其中毛细血管血沉积在特殊的滤纸上。它是一种相对简单的程序，患者可以在家中进行采样。它提供的便利性使其能够用于小分子药物的定量，而近年来，研究旨在开发微采样方法，以促进 mAbs 的 TDM。然而，红细胞压积仍然是阻碍 DBS 在临床实践中更广泛应用的一个障碍。新型分析技术和现代微采样设备的引入可以被认为是解决这一问题的踏脚石。
