Abd-Elgawad Mahfouz M M
National Research Center, Plant Pathology Department, Agricultural and Biological Research Division, El-behooth St., Dokki 12622, Egypt.
Plants (Basel). 2021 Mar 26;10(4):629. doi: 10.3390/plants10040629.
Plant-parasitic and entomopathogenic nematodes (PPNs and EPNs) are key groups in crop production systems. This study aims at optimizing nematode sampling and extraction methods to benefit integrated pest management (IPM) through (a) management of PPNs and (b) use of EPNs. The impacts of these methods on PPNs and EPNs to achieve cost-effective and efficient IPM programs are presented. The common misuses of sampling and extraction methods are discussed. Professionals engaged in IPM should consider sampling the reliability level in the light of the intended goal, location, crop value, susceptibility, nematode species, and available funds. Logical sampling methodology should be expanded to integrate various factors that can recover extra EPN isolates with differential pathogenicity. It should seek for the best EPN-host matching. Merits of repeated baiting for EPN extraction from soil and sieving for PPN recovery from suspensions are presented. Their extraction values may be modelled to quantify the efficiency of nematode separation. The use of proper indices of dispersion to enhance the biocontrol potential of EPNs or save costs in nematicidal applications is ideally compatible with IPM programs. Selecting an extraction method may sometimes require further tests to find the best extraction method of the existing fauna and/or flora. Cons and pros of modern sampling and extraction techniques are highlighted.
植物寄生线虫和昆虫病原线虫（PPNs和EPNs）是作物生产系统中的关键类群。本研究旨在优化线虫采样和提取方法，以通过（a）PPNs的管理和（b）EPNs的使用，使综合虫害管理（IPM）受益。介绍了这些方法对PPNs和EPNs的影响，以实现具有成本效益和高效的IPM计划。讨论了采样和提取方法的常见误用情况。从事IPM的专业人员应根据预期目标、地点、作物价值、敏感性、线虫种类和可用资金来考虑采样的可靠性水平。应扩展合理的采样方法，以整合各种因素，从而获得具有不同致病性的额外EPN分离株。应寻求最佳的EPN-宿主匹配。介绍了从土壤中重复诱捕提取EPNs以及从悬浮液中筛分回收PPNs的优点。可以对它们的提取值进行建模，以量化线虫分离效率。使用适当的分散指数来增强EPNs的生物防治潜力或在杀线虫应用中节省成本，这与IPM计划理想地兼容。选择提取方法有时可能需要进一步测试，以找到针对现有动植物群的最佳提取方法。强调了现代采样和提取技术的优缺点。
