Yang Young-Min, Osawa Kayo, Kitagawa Koichi, Hosoya Samiko, Onishi Reo, Ishii Aya, Shirakawa Toshiro, Hirai Itaru, Kuntaman Kuntaman, Tanimoto Hiroshi, Shigemura Katsumi, Fujisawa Masato
Division of Urology, Department of Organ Therapeutics, Faculty of Medicine, Kobe University Graduate School of Medicine, Kobe, Japan.
Division of Biophysics, Kobe University Graduate School of Health Sciences, Kobe, Japan.
Int J Urol. 2021 Jun;28(6):623-628. doi: 10.1111/iju.14498. Epub 2021 Apr 2.
To compare antibiotic susceptibilities between chromosomal and plasmid bla locations in urinary tract infection-causing extended-spectrum β-lactamases-producing Escherichia coli bla isolated in Indonesia.
A total of 84 strains identified as extended-spectrum β-lactamases-producing E. coli were isolated from patients with urinary tract infection in Indonesia in 2015. Antimicrobial susceptibility tests were performed on these strains using 18 antibiotics, and extended-spectrum β-lactamase bla genes were detected by polymerase chain reaction. Gene localization of bla -positive strains was confirmed by Southern blot hybridization, and epidemiological typing was conducted using multilocus sequence typing.
Of 54 strains harboring the bla gene, 27 showed localization on chromosome, 20 on plasmid, and seven on chromosome and plasmid. Most multilocus sequence typing sequence types of the 27 strains with chromosomal bla were ST405 (25.9%) and ST131 (22.2%) strains, whereas the 20 strains with plasmid-bla were mostly ST410 (55.0%).
Extended-spectrum β-lactamases-producing E. coli bla with plasmid genes show significantly higher resistant rates against piperacillin-tazobactam but lower resistant rates against chloramphenicol compared to chromosomal strains in Indonesian patients with urinary tract infection. Mechanistic investigations will be necessary to advance our knowledge of antimicrobial resistance in urinary tract infection.
比较印度尼西亚分离出的引起尿路感染的产超广谱β-内酰胺酶大肠埃希菌bla在染色体和质粒上的位置的抗生素敏感性。
2015年从印度尼西亚尿路感染患者中分离出84株被鉴定为产超广谱β-内酰胺酶的大肠埃希菌。使用18种抗生素对这些菌株进行药敏试验，并通过聚合酶链反应检测超广谱β-内酰胺酶bla基因。通过Southern印迹杂交确认bla阳性菌株的基因定位，并使用多位点序列分型进行流行病学分型。
在54株携带bla基因的菌株中，27株显示定位于染色体，20株定位于质粒，7株定位于染色体和质粒。27株染色体bla菌株的大多多位点序列分型序列类型为ST405（25.9%）和ST131（22.2%）菌株，而20株质粒bla菌株大多为ST410（55.0%）。
在印度尼西亚尿路感染患者中，与染色体菌株相比，携带质粒基因的产超广谱β-内酰胺酶大肠埃希菌bla对哌拉西林-他唑巴坦的耐药率显著更高，但对氯霉素的耐药率更低。有必要进行机制研究以增进我们对尿路感染中抗菌药物耐药性的了解。
