Institute of Tropical Agriculture and Food Security, Universiti Putra Malaysia, 43400, UPM Serdang, Selangor, Malaysia.
Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400, UPM Serdang, Selangor, Malaysia.
Trop Anim Health Prod. 2021 Apr 3;53(2):242. doi: 10.1007/s11250-021-02683-6.
Previous studies have shown that Mannheimia haemolytica A2 is the principal microorganism causing pneumonic mannheimiosis, a major bacterial respiratory disease among sheep and goats. The effect of this bacteria on the respiratory system is well-established. However, its effect on the reproductive physiology remains unclear. Therefore, this study aimed to determine the alterations in the level of pro-inflammatory cytokines and testosterone hormone post-inoculation with M. haemolytica serotype A2 and its lipopolysaccharide (LPS) endotoxin which were hypothesized to affect the reproductive functions of bucks. Twelve clinically healthy adult male goats were divided equally into three groups. Goats in group 1 were treated with 2 ml of sterile phosphate-buffered saline (PBS) pH 7.0 intranasally (negative control), group 2 with 2 ml of 10 colony-forming unit (CFU) of M. haemolytica serotype A2 intranasally (positive control), and group 3 were treated with 2 ml of lipopolysaccharide extracted from 10 CFU of M. haemolytica serotype A2 intravenously. Following inoculation, blood samples were collected via jugular venipuncture into plain tubes at pre-determined intervals for serum collection to determine the concentration of interleukin (IL)-1β, IL6, tumor necrosis factor (TNF)-α, and testosterone hormone by using commercial ELISA test kits. Results from this study demonstrated that the inoculation of M. haemolytica A2 and its LPS increases the concentration of pro-inflammatory cytokines but decreases the concentration of testosterone hormone in challenged animals at most time points throughout the 56 days experimental period (p < 0.05). This study suggests that the M. haemolytica A2 and its LPS could alter the concentration of pro-inflammatory cytokines and testosterone hormone, which in turn, may negatively affect the reproductive functions of bucks.
先前的研究表明，溶血曼海姆菌 A2 是引起绵羊和山羊肺炎性曼海姆菌病的主要微生物。这种细菌对呼吸系统的影响已经得到证实。然而，其对生殖生理学的影响尚不清楚。因此，本研究旨在确定接种溶血曼海姆菌 A2 血清型及其脂多糖（LPS）内毒素后，促炎细胞因子和睾酮激素水平的变化，这些变化被假设会影响公鹿的生殖功能。12 只临床健康的成年雄性山羊被平均分为三组。第 1 组山羊用 2ml 无菌磷酸盐缓冲盐水（pH7.0）经鼻腔（阴性对照）处理，第 2 组用 2ml 10 个集落形成单位（CFU）的溶血曼海姆菌 A2 经鼻腔（阳性对照）处理，第 3 组用 2ml 从 10 CFU 的溶血曼海姆菌 A2 中提取的脂多糖经静脉内处理。接种后，通过颈静脉穿刺从山羊采集血液样本到普通管中，在预定的间隔时间内采集血清，以使用商业 ELISA 试剂盒测定白细胞介素（IL）-1β、IL6、肿瘤坏死因子（TNF）-α 和睾酮激素的浓度。本研究结果表明，接种溶血曼海姆菌 A2 及其 LPS 会增加大多数时间点的促炎细胞因子浓度，但会降低试验动物在 56 天实验期内的睾酮激素浓度（p<0.05）。本研究表明，溶血曼海姆菌 A2 及其 LPS 可能会改变促炎细胞因子和睾酮激素的浓度，这反过来可能会对公鹿的生殖功能产生负面影响。
