Jia Zhen-Wei, Li Yan, Kong Xiao-Yang, Zhao Hong-Bo, Yang Zhi-Feng, Ye Jing-Wei, Cui Gui-Rong, Luo Jian-Min
Department of Hematology, Handan First Hospital, Handan 056002, Hebei Province, China.
Department of Hematology, Handan First Hospital, Handan 056002, Hebei Province, China,E-mail:
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2021 Apr;29(2):494-499. doi: 10.19746/j.cnki.issn.1009-2137.2021.02.029.
To investigate the effect of long non-coding RNA-TUC338 on the proliferation and migration of lymphoma cells.
The expression of TUC338 in different lymphoma cells was detected by fluorescence quantitative PCR, cell proliferation by sulforhodamine B (SRB) assay, migration of lymphoma cells by transwell assay, and protein expression in PI3K/AKT signaling pathway by Western blot.
The expression levels of TUC338 in lymphoma cells Daudi, U937, BC-3, and Raji significantly increased in comparison with human normal T lymphocytes H9 (t=13.277, 10.103, 16.200, and 26.687, P=0.002, 0.005, 0.001, and 0.000). Compared with NC-siRNA group, the number of cells crossing the chamber of TUC338-siRNA group was significantly reduced (t=30.508, P=0.000), the protein expression levels of p-PI3K and p-AKT significantly decreased (t=16.872 and 18.371, P=0.000 and 0.000), and OD absorbance values at 24 h, 48 h, and 72 h were significantly lower (P<0.05).
The expression of TUC338 significantly increases in lymphoma cells, and silence of TUC338 effectively inhibits the activation of PI3K/AKT signaling pathway, thereby inhibiting the proliferation and migration of lymphoma cells, which has a potential application value in diagnosis and treatment of lymphoma.
探讨长链非编码RNA-TUC338对淋巴瘤细胞增殖和迁移的影响。
采用荧光定量PCR检测不同淋巴瘤细胞中TUC338的表达，用磺酰罗丹明B（SRB）法检测细胞增殖，用Transwell法检测淋巴瘤细胞迁移，用蛋白质印迹法检测PI3K/AKT信号通路中的蛋白表达。
与人类正常T淋巴细胞H9相比，淋巴瘤细胞Daudi、U937、BC-3和Raji中TUC338的表达水平显著升高（t=13.277、10.103、16.200和26.687，P=0.002、0.005、0.001和0.000）。与NC-siRNA组相比，TUC338-siRNA组穿过小室的细胞数量显著减少（t=30.508，P=0.000），p-PI3K和p-AKT的蛋白表达水平显著降低（t=16.872和18.371，P=0.000和0.000），24 h、48 h和72 h时的OD吸光度值显著降低（P<0.05）。
TUC338在淋巴瘤细胞中的表达显著增加，沉默TUC338可有效抑制PI3K/AKT信号通路的激活，从而抑制淋巴瘤细胞的增殖和迁移，在淋巴瘤的诊断和治疗中具有潜在应用价值。
